Document Detail

Human oocyte cryopreservation: comparison between slow and ultrarapid methods.
MedLine Citation:
PMID:  19712551     Owner:  NLM     Status:  MEDLINE    
The success of reproductive technologies is facilitated by the cryopreservation of embryos and gametes. In Italy, where legislation prohibits zygote and embryo cryopreservation, clinics have extensively introduced oocyte cryopreservation. Two different strategies of oocyte cryopreservation are available: slow freezing or ultrarapid cooling (vitrification). Although the results are very encouraging with both methods, there is still controversy regarding both the procedure itself and the most suitable method to use. This study reports the routine application of the two different oocyte cryopreservation methods in programmes running in two consecutive periods. The study centre carried out 286 thawing cycles for a total of 1348 thawed oocytes cryopreserved by the slow-freezing method and 59 warming cycles for a total of 285 warmed oocytes cryopreserved by vitrification. Comparison of the outcomes obtained with the slow-freezing method versus vitrification in women who underwent IVF for infertility showed survival, fertilization, pregnancy and implantation rates of 57.9% versus 78.9% (P < 0.0001), 64.6% versus 72.8% (P = 0.027), 7.6% versus 18.2% (P = 0.021) and 4.3% versus 9.3% (P = 0.043) respectively. These results suggest that oocyte vitrification is associated with a better outcome than the slow-freezing method.
R Fadini; F Brambillasca; M Mignini Renzini; M Merola; R Comi; E De Ponti; M B Dal Canto
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  19     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-28     Completed Date:  2009-11-09     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  171-80     Citation Subset:  IM    
BIOGENESI Reproductive Medicine Centre, Istituti Clinici Zucchi, V. Zucchi, 24-Monza, Italy.
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MeSH Terms
Cryopreservation / methods*
Erratum In:
Reprod Biomed Online. 2009 Nov;19(5):757

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