Document Detail


Human olfactory epithelium in normal aging, Alzheimer's disease, and other neurodegenerative disorders.
MedLine Citation:
PMID:  1723988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By use of immunohistochemistry, we characterized the molecular phenotype of human olfactory epithelial (OE) cells and assessed the nature of the dystrophic olfactory neurites described initially in Alzheimer's disease (AD). Keratin 8 was present in all classes of OE cells. Sustentacular cells lacked other cell type specific polypeptides and were distinguished from neurons and basal cells because the latter two classes of OE cells expressed neural cell adhesion molecules (N-CAMs) and microtubule associated proteins (MAPs), i.e., MAP5. Basal cells expressed nerve growth factor receptors (NGFRs), which distinguished them from olfactory neurons. Unlike their perikarya, olfactory axons expressed vimentin and GAP-43, but not peripherin or neurofilament (NF) proteins. Olfactory nerves were distinguished from other axons because the latter were positive for all three NF subunits and peripherin, in addition to vimentin and GAP-43. Dystrophic neurites in the OE were GAP-43 positive, but they also expressed proteins that were not detected in normal olfactory nerves (i.e., synaptophysin, MAP2, tau, peripherin, NF proteins). Further, rare NF positive olfactory neurons gave rise to NF positive dystrophic neurites. These neurites were present in all 11 AD cases, 11 of 14 subjects with other neurodegenerative diseases, and 6 of 8 neurologically normal adult controls, but no dystrophic neurites were seen in 9 fetal and neonatal cases. We conclude that the molecular phenotype of different human OE cells is distinct and that dystrophic olfactory neurites occur very frequently in neurologically normal adults. The relevance of these neurites to aging or specific disease processes remains speculative.
Authors:
J Q Trojanowski; P D Newman; W D Hill; V M Lee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  310     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1992-03-20     Completed Date:  1992-03-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  365-76     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104-4283.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aging
Alzheimer Disease / pathology*
Cell Adhesion Molecules, Neuronal / analysis
Epithelial Cells
Epithelium / pathology,  ultrastructure
Female
Humans
Immunohistochemistry
Infant
Infant, Newborn
Infant, Premature
Keratins / analysis
Male
Microscopy, Electron
Microtubule-Associated Proteins / analysis
Middle Aged
Nerve Growth Factors / metabolism
Nervous System Diseases / pathology*
Neurites / ultrastructure
Olfactory Mucosa / growth & development,  innervation*,  pathology
Receptors, Cell Surface / analysis
Receptors, Nerve Growth Factor
Vimentin / analysis
Grant Support
ID/Acronym/Agency:
AG-09215/AG/NIA NIH HHS; CA-36245/CA/NCI NIH HHS; HD-26979/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules, Neuronal; 0/Microtubule-Associated Proteins; 0/Nerve Growth Factors; 0/Receptors, Cell Surface; 0/Receptors, Nerve Growth Factor; 0/Vimentin; 68238-35-7/Keratins

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