Document Detail

Human nucleus pulposus cells significantly enhanced biological properties in a coculture system with direct cell-to-cell contact with autologous mesenchymal stem cells.
MedLine Citation:
PMID:  19953600     Owner:  NLM     Status:  MEDLINE    
Activated nucleus pulposus (NP) cells can be reinserted into the disc to inhibit intervertebral disc degeneration. Experimental studies in animals showed that using a coculture system with direct cell-to-cell contact with mesenchymal stem cells (MSCs) significantly upregulated the biological activity of NP cells. The purpose of this study is to determine whether this activation of NP cells by autologous MSCs is applicable to human cells in vitro. Human NP tissue was obtained from surgical specimens and MSCs from bone marrow of 10 subjects. Six-well culture plates and inserts were used for culture; 1.0x10(4) NP cells were seeded onto each insert and incubated alone, in standard coculture with 1.0x10(4) MSCs, or cocultured with direct cell-to-cell contact. NP cell proliferation, DNA synthesis, and proteoglycan (PG) synthesis were evaluated. Chromosome abnormalities in the activated NP cells and tumorigenesis of the cells were evaluated in an additional 10 patients by microscopic examination for segmented cells and histological assessment of activated cells transplanted into nude mice. Cell proliferation, DNA synthesis, and PG synthesis were significantly upregulated. The positive effects of the coculture system with direct cell-to-cell contact seen in animal studies were also confirmed in human cells. Chromosome abnormalities and tumorigenesis were not observed in the activated NP cells. In conclusion, a coculture system with direct cell-to-cell contact demonstrated a significant positive effect, enhancing the biological properties of human NP cells, as it did in animal models. These results should prove useful for conducting trials leading to the clinical use of activated NP cell transplantation.
Takuya Watanabe; Daisuke Sakai; Yukihiro Yamamoto; Toru Iwashina; Kenji Serigano; Futoshi Tamura; Joji Mochida
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  28     ISSN:  1554-527X     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  623-30     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Orthopaedic Research Society.
Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
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MeSH Terms
Bone Marrow Cells / cytology
Cell Communication / physiology*
Cell Division / physiology
Cell Transplantation / methods*
Cells, Cultured
Chromosome Aberrations
Coculture Techniques / methods*
Intervertebral Disk / cytology*
Intervertebral Disk Degeneration / pathology*,  surgery
Magnetic Resonance Imaging
Mesenchymal Stem Cells / cytology*
Mice, Nude
Middle Aged
Neoplasms / genetics,  pathology,  prevention & control
Proteoglycans / metabolism
Spinal Fractures / pathology,  surgery
Spondylolysis / pathology,  surgery
Transplantation, Autologous
Young Adult
Reg. No./Substance:

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