Document Detail


Human neoplastic cell line distribution, immunoprecipitation and immunohistopathological study of a gp200 cell surface glycoprotein (LCA) detected by a monoclonal antibody elicited with an ALL cell line.
MedLine Citation:
PMID:  2963236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The antigen recognized by a newly produced monoclonal antibody (bra55; IgG1) elicited by the non-T, non-B acute lymphoblastic leukemia cell line REH 6, was expressed on all examined hemopoietic neoplastic cell lines (including non-T, non-B, T, B and myeloid leukemia cell lines), but not on examined nonhemopoietic human tumor cell lines (such as carcinoma, sarcoma, melanoma and neuroblastoma cell lines), as demonstrated by indirect immunofluorescence and enzyme-linked immunoassay. Specific immunoprecipitation of 125I-lacto-peroxidase radioiodinated cell surface proteins and sodium metaperiodate/tritiated sodium borohydride 3H-radiolabeled cell surface sialoglycoproteins followed by electrophoretic analysis (SDS-PAGE) demonstrated that the immunoprecipitated antigen is a cell surface 200 kDa sialoglycoprotein (on the non-T, non-B ALL cell line REH 6), with variation in its electrophoretic mobility (in the Mr range of 170,000-210,000) on different examined cell lines. These properties are characteristic for the leukocyte common antigen (LCA, T200). Immunoperoxidase staining of several normal and malignant tissues, as well as some nonhemopoietic tumor tissues confirmed the type of antigen tissue distribution pattern characteristic for LCA.
Authors:
B Chorváth; K Poláková; J Sedlák; J Matoska; J Duraj; I Plesková; M Karpatová; M Grófová
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Neoplasma     Volume:  34     ISSN:  0028-2685     ISO Abbreviation:  Neoplasma     Publication Date:  1987  
Date Detail:
Created Date:  1988-03-09     Completed Date:  1988-03-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0377266     Medline TA:  Neoplasma     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  685-92     Citation Subset:  IM    
Affiliation:
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal*
Antigens, CD45
Cell Line
Fluorescent Antibody Technique
Histocompatibility Antigens / analysis*
Humans
Immunoenzyme Techniques
Leukemia, Lymphoid / immunology*
Membrane Glycoproteins / analysis*
Neoplasms / analysis*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Histocompatibility Antigens; 0/Membrane Glycoproteins; EC 3.1.3.48/Antigens, CD45

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