Document Detail


Human monoclonal antibodies for the immunological characterization of a highly conserved protein domain of the hepatitis C virus glycoprotein E1.
MedLine Citation:
PMID:  7544250     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although both envelope glycoproteins of the hepatitis C virus, E1 and E2/NS1, show a high degree of sequence variation, the E1 protein includes a well conserved domain, which may be functionally important. We have analysed the human B cell response to a peptide fragment from amino acid residues 314-330 (EP3) covering the central conserved sequence of this domain. Anti-hepatitis C virus-positive blood donors were screened for anti-EP3 antibodies with an ELISA based on immobilized peptide. Thirty out of 92 (32%) RIBA-confirmed donors displayed a significant antibody response to EP3. From three of these blood donors we established four anti-EP3-producing heterohybridoma cell lines: Ul/F30 and Ul/F31 produced IgM-kappa, whereas Ul/F32 and Ul/F33 secreted the isotypes IgG1-lambda and IgG1-kappa, respectively. Epitope analysis with overlapping nonapeptides suggests the existence of different antigenic determinants within the EP3 fragment. Although both IgG antibodies Ul/F32 and Ul/F33 have dissociation constants to the peptide of approximately 10(-9) M, binding to recombinant E1 protein expressed in COS-7 cells was different. Only Ul/F33 detected envelope protein of approximately 24-35 kD in Western blot. This human MoAb will be useful for further investigations on the hepatitis C virus glycoprotein E1.
Authors:
K Siemoneit; M da S Cardoso; K Koerner; A Wölpl; B Kubanek
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  101     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-22     Completed Date:  1995-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  278-83     Citation Subset:  IM    
Affiliation:
German Red Cross Blood Bank, Ulm.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Antibodies, Monoclonal / immunology
Antibody Specificity
B-Lymphocytes / drug effects,  immunology
Conserved Sequence*
Hepatitis Antibodies / blood,  immunology*
Hepatitis C / blood
Hepatitis C Antibodies
Humans
Immunoblotting
Immunoglobulin G / immunology
Leukocytes, Mononuclear / immunology
Lymphocyte Activation / immunology
Molecular Sequence Data
Peptide Fragments / immunology
Viral Envelope Proteins / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/E1 protein, Hepatitis C virus; 0/Hepatitis Antibodies; 0/Hepatitis C Antibodies; 0/Immunoglobulin G; 0/Peptide Fragments; 0/Viral Envelope Proteins
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