Document Detail


Human monoclonal antibodies: analysis of two antibodies derived from lymphocytes of a patient with acute rheumatic fever.
MedLine Citation:
PMID:  1811117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human monoclonal antibodies were produced by fusion of peripheral blood lymphocytes from a patient with acute rheumatic fever, with the HGPRT-non-secreting murine (Balb-c) cell line SP2/0Ag14. Heterohybridomas were selected by screening against rheumatic fever-associated group A streptococci using an ELISA, and against paraffin wax-embedded human heart sections using an immunoperoxidase technique. Two human IgM monoclonal antibodies were selected for further analysis by Western blotting and ELISA. Both antibodies demonstrated multispecificity by immunoblotting and ELISA. One of the monoclonals bound to 48 kD and 83 kD bands common to group A streptococcal and heart antigen preparations. Both human monoclonal antibodies bound to a 43 kD constituent band common to human heart and sarcolemma membrane extract. Inhibition studies performed using a competitive solid phase immunoassay confirmed shared epitopes between group A streptococci and human heart. The significance of these monoclonal antibodies to the pathogenesis of rheumatic fever is uncertain.
Authors:
E J Hughes; Q Eichbaum; D W Beatty
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical laboratory sciences     Volume:  48     ISSN:  0308-3616     ISO Abbreviation:  Med Lab Sci     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1992-05-29     Completed Date:  1992-05-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7609161     Medline TA:  Med Lab Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  244-55     Citation Subset:  IM    
Affiliation:
Institute of Child Health, Red Cross War Memorial Children's Hospital, Cape Town, South Africa.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Antibodies, Bacterial / analysis*
Antibodies, Monoclonal / analysis*
Cross Reactions / immunology
Humans
Hybridomas / immunology
Immunologic Techniques
Lymphocytes / immunology
Myocardium / immunology*
Rheumatic Heart Disease / immunology*
Streptococcus pyogenes / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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