Document Detail


Human and monkey prolactin and growth hormone: separation of polymorphic forms by isoelectric focusing.
MedLine Citation:
PMID:  227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The feasibility of using isoelectric focusing for the separation of primate pituitary growth hormone from prolactin and for the characterization of polymorphic forms of these hormones was explored. In a pH 3--10 gradient, extracts of both human and cynomolgus monkey pituitaries were each resolved into 4 growth hormone components and at least 3 prolactin components, as shown by radioimmunoassay. In narrower gradients (of 2--3 pH units) greater resolution was achieved; the principal growth hormone components were well separated from the principal prolactin components but there was overlapping of some minor components. A partially purified human prolactin preparation was found to contain 4 prolactin components, one of which had a prolactin/growth hormone ratio of 760. Clinical grade human growth hormone was also resolved into at least 5 prolactin and 5 growth hormone components, many of which had higher pI values than those found in pituitary extract. Under the conditions used, both growth hormone and prolactin were found to be polymorphic with respect to isoelectric point. Some of the human prolactin components were found to contain less than 0.2% growth hormone by radioimmunoassay. Monkey growth hormone containing 0.01% prolactin was isolated. These findings demonstrate that isoelectric focusing is useful for the preparation of both growth hormone and prolactin which are essentially free of one another. Furthermore, the polymorphic forms were repeatedly found in preparations obtained by several methods and from 2 different species, suggesting that these forms are not artifacts.
Authors:
B C Hummel; G M Brown; P Hwang; H G Friesen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  97     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1976-02-09     Completed Date:  1976-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  855-67     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Growth Hormone / isolation & purification*
Haplorhini
Humans
Hydrogen-Ion Concentration
Isoelectric Focusing
Macaca fascicularis
Pituitary Gland / analysis*
Prolactin / isolation & purification*
Radioimmunoassay
Chemical
Reg. No./Substance:
9002-62-4/Prolactin; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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