| Human mid-gestation amniotic fluid contains interleukin-16 bioactivity. | |
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MedLine Citation:
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PMID: 19278422 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CD4-positive cells are detectable in the human fetal gastrointestinal tract from 11 weeks of gestation. Interleukin-16 (IL-16) is a chemoattractant for CD4(+) cells and, via fetal swallowing of amniotic fluid, could mediate the influx of CD4(+) cells into the fetal gut. We have shown that IL-16 was detectable in human amniotic fluid at 16-18 weeks of gestation (mid-pregnancy) but was not detectable at term (late pregnancy; > 37 weeks of gestation). Similarly, mid-pregnancy, but not late pregnancy, amniotic fluid contained chemotactic activity for CD4(+) T cells, this activity was reduced by 58% in the presence of a neutralizing anti-IL-16 antibody. The levels of IL-16 in fetal plasma at 16-24 weeks of gestation were very high, and decreased significantly by 25-36 weeks but at > 37 weeks remained significantly higher than adult levels. IL-16 transcripts were detectable in whole tissue extracts of fetal gut, skin and placenta but not in amniocytes, and IL-16 immunoreactivity was detectable in cells within the lamina propria of the fetal gut and within the skin, where it was associated with the basement membrane. Neither IL-16 levels nor chemotactic activity for CD4(+) T cells in mid-pregnancy amniotic fluid was related to atopic outcomes at 1 year of age. IL-16 might have an important role in the early development of the human immune system and/or in regulating fetal and maternal immunological responsiveness during pregnancy. |
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Authors:
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Catherine A Thornton; Judith A Holloway; Janis K Shute; John W Holloway; Norma D Diaper; John O Warner |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunology Volume: 126 ISSN: 1365-2567 ISO Abbreviation: Immunology Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-12 Completed Date: 2009-05-14 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 0374672 Medline TA: Immunology Country: England |
Other Details:
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Languages: eng Pagination: 543-51 Citation Subset: IM |
Affiliation:
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Institute of Life Science, School of Medicine, Swansea University, Swansea. c.a.thornton@swansea.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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immunology* CD4-Positive T-Lymphocytes / immunology Chemotaxis, Leukocyte / immunology Fetus / immunology Gestational Age Humans Hypersensitivity, Immediate / embryology, immunology Infant Interleukin-16 / metabolism* Intestines / embryology, immunology Skin / embryology, immunology |
| Grant Support | |
ID/Acronym/Agency:
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HL-61858/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-16 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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