Document Detail


Human metapneumovirus in infants and young children in Thailand with lower respiratory tract infections; molecular characteristics and clinical presentations.
MedLine Citation:
PMID:  16183133     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human metapneumovirus (hMPV) is a recently identified Paramyxovirus. The clinical features and molecular characteristics of hMPV in Asian populations have so far remained obscure. OBJECTIVE: The present study was designed to investigate the prevalence of hMPV in infants and young children presented with acute lower respiratory tract infection (ALRI) and to identify the molecular characteristics and clinical presentations. METHODS: There were 236 nasopharyngeal secretions (NPs) collected from infants and children presented with ALRI at King Chulalongkorn Memorial hospital between March 2001 and September 2003. Reverse transcriptase-polymerase chain reaction (RT-PCR) applying specific primers was done to identify hMPV and hRSV. Phylogenetic analysis of hMPV N, F and L genes was also performed. RESULTS: Of the 220 (236) infants and young children tested, positive results were found in 12 specimens (5.4%). The mean age of children with hMPV infections was 22+/-11 months. They mostly presented with fever with cough (100%) and upper respiratory tract symptoms (10/12, 83%). Eleven of twelve infants (92%) were hospitalized. Additionally, phylogenetic analysis identified two distinct lineages of hMPV. CONCLUSION: Our results demonstrated the prevalence, molecular characterization and clinical spectrum of hMPV infection in infants and young children presented with lower respiratory tract infections in Thailand.
Authors:
Rujipat Samransamruajkit; Wanida Thanasugarn; Nuanchan Prapphal; Apiradee Theamboonlers; Yong Poovorawan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-23
Journal Detail:
Title:  The Journal of infection     Volume:  52     ISSN:  1532-2742     ISO Abbreviation:  J. Infect.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-03     Completed Date:  2007-07-20     Revised Date:  2007-12-14    
Medline Journal Info:
Nlm Unique ID:  7908424     Medline TA:  J Infect     Country:  England    
Other Details:
Languages:  eng     Pagination:  254-63     Citation Subset:  IM    
Affiliation:
Respiratory and Critical Care Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY158463;  AY158464;  AY158465;  AY550148;  AY550149;  AY550150;  AY550151;  AY550152;  AY550153;  AY550154;  AY550155;  AY550156;  AY550157;  AY550158;  AY550159;  AY550160;  AY550161;  AY550162;  AY550163;  AY550164;  AY550165;  AY550166;  AY550167;  AY550168;  AY550169;  AY550170;  AY550171;  AY550172;  AY550173;  AY550174;  AY550175
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Child, Preschool
DNA Primers / chemistry
DNA, Viral / chemistry
Female
Humans
Infant
Male
Metapneumovirus / classification,  genetics*,  isolation & purification,  pathogenicity*
Molecular Sequence Data
Nasopharynx / virology
Paramyxoviridae Infections / diagnosis,  epidemiology*,  virology
Phylogeny
Prevalence
Respiratory Syncytial Viruses / genetics,  isolation & purification
Respiratory Tract Infections / diagnosis,  epidemiology*,  virology
Reverse Transcriptase Polymerase Chain Reaction / methods
Thailand / epidemiology
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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