Document Detail

Human mesenchymal stromal cells improve scar thickness without enhancing cardiac function in a chronic ischaemic heart failure model.
MedLine Citation:
PMID:  22361124     Owner:  NLM     Status:  MEDLINE    
Few data address the role of human mesenchymal stromal cells (MSCs) in the management of chronic ischaemic heart failure. We assessed their effect in immune-deficient animals. MSCs were cultured from bone marrow of human volunteers. Non-obese diabetes severe combined immunodeficiency (NOD/SCID) gamma null mice were randomly assigned to intramyocardial injection of human MSCs or phosphate-buffered saline 4 weeks after induction of acute myocardial infarction (MI). Echocardiography was performed 4 weeks after MI and 1 and 4 weeks after injection. Donor cell chimerism was assessed by DNA for human Alu sequences 2 and 4 weeks after injection. Histological assessment and quantification of neovascularization were determined 4 weeks after treatment. Donor MSCs at frequencies of 0.006 and 0.001% were present 2 and 4 weeks after cell injection, respectively. The infarcted ventricular wall was significantly thicker in the cohort receiving MSCs compared with control mice. There was no difference in fractional shortening, left ventricular dimensions or scar area between the groups. Small vessel density was also similar between the groups. Human MSCs increased the thickness of the infarcted ventricular wall without improving cardiac function in this chronic ischaemic heart failure model. Further studies are required to assess the benefit of MSCs in this setting.
Victor Dayan; Gustavo Yannarelli; Paola Filomeno; Armand Keating
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-02-22
Journal Detail:
Title:  Interactive cardiovascular and thoracic surgery     Volume:  14     ISSN:  1569-9285     ISO Abbreviation:  Interact Cardiovasc Thorac Surg     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-18     Completed Date:  2012-08-02     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101158399     Medline TA:  Interact Cardiovasc Thorac Surg     Country:  England    
Other Details:
Languages:  eng     Pagination:  516-20     Citation Subset:  IM    
Cell Therapy Program, Princess Margaret Hospital, University Health Network, Toronto, Canada.
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MeSH Terms
Biological Markers / metabolism
Cell Proliferation
Cells, Cultured
Chronic Disease
Cicatrix / metabolism,  pathology*
Disease Models, Animal
Heart Failure / genetics,  metabolism,  physiopathology,  surgery*,  ultrasonography
Interleukin Receptor Common gamma Subunit / deficiency,  genetics
Mesenchymal Stem Cell Transplantation*
Mice, Inbred NOD
Mice, Knockout
Mice, SCID
Myocardial Infarction / genetics,  metabolism,  physiopathology,  surgery*,  ultrasonography
Myocardium / metabolism,  pathology*
Neovascularization, Physiologic
Time Factors
Ventricular Function, Left
Ventricular Remodeling*
Reg. No./Substance:
0/Biological Markers; 0/Il2rg protein, mouse; 0/Interleukin Receptor Common gamma Subunit

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