Document Detail

Human mesenchymal stem cells do not differentiate into cardiomyocytes in a cardiac ischemic xenomodel.
MedLine Citation:
PMID:  16581700     Owner:  NLM     Status:  MEDLINE    
AIM: As the capability of human mesenchymal stem cells (hMSC) to engraft, differentiate and improve myocardial function cannot be studied in humans, exploration was performed in a xenomodel. METHODS: The rats were divided into three groups depending on the type of rats used (Rowett nude (RNU) or Fischer rats +/- immunosuppression). Different groups were treated with intramyocardial injection of hMSC (1-2 million) either directly or three days after ligation of the left anterior descending artery (LAD). Myocardial function was investigated by echocardiography. The hMSC were identified with fluorescence in situ hybridization and myocardial differentiation was assessed by immunohistochemistry. RESULTS: When hMSC were injected directly after LAD ligation they could be identified in half (8/16) of the RNU rats (without immunosuppression) at 4 weeks. When injected 3 days after LAD ligation in immunosuppressed RNU rats they were identified in all (6/6) rats at 6 weeks. The surviving hMSC showed signs of differentiation into fibroblasts. No cardiomyocyte differentiation was observed. There was no difference in myocardial function in treated animals compared to controls. CONCLUSIONS: The hMSC survived in this xenomodel up to 6 weeks. However, hMSC required implantation into immunoincompetent animals as well as immunosuppression to survive, indicating that these cells are otherwise rejected. Furthermore, these cells did not differentiate into cardiomyocytes nor did they improve heart function in this xenomodel.
Karl-Henrik Grinnemo; Agneta Månsson-Broberg; Katarina Leblanc; Matthias Corbascio; Eva Wärdell; Anwar J Siddiqui; Xiaojin Hao; Christer Sylvén; Göran Dellgren
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of medicine     Volume:  38     ISSN:  0785-3890     ISO Abbreviation:  Ann. Med.     Publication Date:  2006  
Date Detail:
Created Date:  2006-04-03     Completed Date:  2006-08-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8906388     Medline TA:  Ann Med     Country:  Sweden    
Other Details:
Languages:  eng     Pagination:  144-53     Citation Subset:  IM    
Department of Cardiothoracic Surgery and Anaesthesiology, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden.
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MeSH Terms
Disease Models, Animal
Graft Survival
In Situ Hybridization, Fluorescence
Lymphocyte Culture Test, Mixed
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / immunology*
Myocardial Infarction / pathology,  therapy*
Myocardial Ischemia / pathology
Myocardium* / pathology
Myocytes, Cardiac / immunology*
Rats, Inbred F344
Rats, Nude

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