Document Detail

Human malignant melanoma cell line (HMV-II) for isolation of influenza C and parainfluenza viruses.
MedLine Citation:
PMID:  2166075     Owner:  NLM     Status:  MEDLINE    
HMV-II, a human malignant melanoma cell line, was compared with other cell lines (MDCK, Vero, and LLC-MK2) and primary cultures of monkey kidney (PMK) cells for the isolation and quantification of influenza and parainfluenza viruses. HMV-II cells were superior to MDCK and LLC-MK2 cells in quantification of the influenza C virus and were used successfully in the isolation of the virus from clinical specimens. The HMV-II cell line was also more sensitive for isolating parainfluenza viruses from clinical specimens than were Vero and PMK cells; there was, however, no significant difference in the quantification of the viruses among these cultures. As far as influenza A and B viruses were concerned, the HMV-II cell line was significantly less sensitive than MDCK cells, and no virus was isolated from clinical specimens with HMV-II cells. Thus, HMV-II cells are useful for the isolation of influenza C and parainfluenza viruses as an alternative to embryonated hen's eggs and PMK cells.
H Moriuchi; T Oshima; H Nishimura; K Nakamura; N Katsushima; Y Numazaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  28     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-09-12     Completed Date:  1990-09-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1147-50     Citation Subset:  IM    
Virus Center, Sendai National Hospital, Japan.
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MeSH Terms
Chick Embryo
Cytopathogenic Effect, Viral
Erythrocytes / microbiology
Guinea Pigs
Influenzavirus C / isolation & purification*
Orthomyxoviridae / isolation & purification*
Orthomyxoviridae Infections / complications
Paramyxoviridae Infections / complications
Respirovirus / isolation & purification*
Sensitivity and Specificity
Tumor Cells, Cultured
Virus Cultivation*

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