| Human luteinized granulosa cells secrete apoB100-containing lipoproteins. | |
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MedLine Citation:
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PMID: 20407020 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thus far, liver, intestine, heart, and placenta have been shown to secrete apolipoprotein (apo)B-containing lipoproteins. In the present study, we first investigated lipoproteins in human follicular fluid (FF), surrounding developing oocytes within the ovary, as well as in corresponding plasma samples (n = 12). HDL cholesterol within FF correlated well with plasma HDL cholesterol (r = 0.80, P < 0.01), whereas VLDL cholesterol did not, indicating that VLDL in FF might originate directly from the granulosa cells producing FF. Primary human granulosa cells expressed apoB, microsomal triglyceride transfer protein, and apoE, but not the apoB-editing enzyme apobec-1. Using (3)H-leucine, we show that granulosa cells secrete apoB100-containing lipoproteins and that secretion can be stimulated by adding oleate to the medium (+83%). With electron microscopy, apoB-containing lipoproteins within the secretory pathway of human granulosa cells were directly visualized. Finally, we found a positive relationship between apoB levels in FF and improved fertility parameters in a population of 27 women undergoing in vitro fertilization. This study demonstrates that human granulosa cells assemble and secrete apoB100-containing lipoproteins, thereby identifying a novel cell type equipped with these properties. These results might have important implications for female infertility phenotypes as well as for the development of drugs targeting the VLDL production pathway. |
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Authors:
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Thomas Gautier; Steffi Becker; Véronique Drouineaud; Franck Ménétrier; Paul Sagot; Jerzy-Roch Nofer; Sören von Otte; Laurent Lagrost; David Masson; Uwe J F Tietge |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-20 |
Journal Detail:
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Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-15 Completed Date: 2010-10-20 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 2245-52 Citation Subset: IM |
Affiliation:
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Faculté de Médecine, INSERM UMR866 Lipides, Nutrition, Cancer, Dijon, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apolipoprotein B-100
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genetics,
secretion* Carrier Proteins / genetics Cholesterol, HDL / blood Cholesterol, VLDL / chemistry Female Fertility Follicular Fluid / cytology Gene Expression Regulation / drug effects Granulosa Cells / drug effects, metabolism, secretion*, ultrastructure Hep G2 Cells Humans Luteinization* Microscopy, Electron Oleic Acid / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein B-100; 0/Carrier Proteins; 0/Cholesterol, HDL; 0/Cholesterol, VLDL; 0/microsomal triglyceride transfer protein; 112-80-1/Oleic Acid |
| Comments/Corrections | |
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