Document Detail


Human kallikrein hK2 has low kininogenase activity while prostate-specific antigen (hK3) has none.
MedLine Citation:
PMID:  9428664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present paper, we determined the kinin-releasing activity of human prostatic kallikrein hK2 and compared it to one of the kallikreins hK1 and prostate specific antigen (hK3). Kinin-like substances active on the rabbit jugular vein were progressively produced when nanomolar concentrations of hK2 were incubated with heated plasma. However in these experiments, hK1 appeared much more potent than hK2 while hK3 was totally inactive. When hK2 was incubated with purified high molecular weight kininogen, several peptides were generated as shown by the analysis on C18 reverse-phase HPLC. Kinin activity was localized exclusively in a small peak having an elution time identical to that of bradykinin while the only important peak obtained with hK1 corresponded to Lys-bradykinin. Finally, the rate of kinin production of hK2 was found to be more than a thousandfold lower than that of hK1. These experiments show that kallikreins hK2 has only a low kininogenase activity. However, it is not excluded that some of the peptides produced by hK2 action could have other types of biological activity.
Authors:
D Deperthes; F Marceau; G Frenette; C Lazure; R R Tremblay; J Y Dubé
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1343     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-30     Completed Date:  1998-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  102-6     Citation Subset:  IM    
Affiliation:
Laboratory of Hormonal Bioregulation, CHUL Research Center and Laval University, Sainte-Foy, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Enzyme Activation
Humans
Kallikreins / metabolism*
Male
Prostate-Specific Antigen / metabolism*
Rabbits
Chemical
Reg. No./Substance:
EC 3.4.21.-/Kallikreins; EC 3.4.21.77/Prostate-Specific Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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