| Human kallikrein hK2 has low kininogenase activity while prostate-specific antigen (hK3) has none. | |
| | |
MedLine Citation:
|
PMID: 9428664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the present paper, we determined the kinin-releasing activity of human prostatic kallikrein hK2 and compared it to one of the kallikreins hK1 and prostate specific antigen (hK3). Kinin-like substances active on the rabbit jugular vein were progressively produced when nanomolar concentrations of hK2 were incubated with heated plasma. However in these experiments, hK1 appeared much more potent than hK2 while hK3 was totally inactive. When hK2 was incubated with purified high molecular weight kininogen, several peptides were generated as shown by the analysis on C18 reverse-phase HPLC. Kinin activity was localized exclusively in a small peak having an elution time identical to that of bradykinin while the only important peak obtained with hK1 corresponded to Lys-bradykinin. Finally, the rate of kinin production of hK2 was found to be more than a thousandfold lower than that of hK1. These experiments show that kallikreins hK2 has only a low kininogenase activity. However, it is not excluded that some of the peptides produced by hK2 action could have other types of biological activity. |
| | |
Authors:
|
D Deperthes; F Marceau; G Frenette; C Lazure; R R Tremblay; J Y Dubé |
Related Documents
:
|
15135584 - Modelling effect of physical and chemical parameters on heat inactivation kinetics of h... 2589024 - Establishment of a monoclonal antibody against senile plaques and its application for i... 20652644 - Content variations of the tomato saponin esculeoside a in various processed tomatoes. 16965144 - Comparative study of cryogen spray cooling with r-134a and r-404a: implications for las... 11539534 - Inhalation risk in low-gravity spacecraft. 17218444 - Milnacipran, a serotonin and norepinephrine reuptake inhibitor, induces appetite-suppre... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1343 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1997 Nov |
Date Detail:
|
Created Date: 1998-01-30 Completed Date: 1998-01-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 102-6 Citation Subset: IM |
Affiliation:
|
Laboratory of Hormonal Bioregulation, CHUL Research Center and Laval University, Sainte-Foy, Quebec, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Enzyme Activation Humans Kallikreins / metabolism* Male Prostate-Specific Antigen / metabolism* Rabbits |
| Chemical | |
Reg. No./Substance:
|
EC 3.4.21.-/Kallikreins; EC 3.4.21.77/Prostate-Specific Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Substrate induced reactivation of spinach ribulose-1,5-bisphosphate carboxylase/oxygenase denatured ...
Next Document: Three-state kinetic analysis of Chinese hamster dihydrofolate reductase unfolding by guanidine hydro...