Document Detail

Human islet isolation--a prospective randomized comparison of pancreatic vascular perfusion with hyperosmolar citrate or University of Wisconsin solution.
MedLine Citation:
PMID:  8212148     Owner:  NLM     Status:  MEDLINE    
University of Wisconsin solution has become the most commonly used vascular perfusate during multiorgan donation world-wide. In the UK however, hyperosmolar citrate remains in common use. The purpose of this prospective randomized study was to compare the effect of systemic perfusion with UW or HOC on subsequent islet yield and purification for pancreata with short cold ischemic times. Seven pancreata were randomized to each group, with the donor age, pancreas weight, and period of cold ischemia being similar in both. Perfusion with UW was shown to inhibit collagenase digestion, and a higher concentration of this enzyme was needed to achieve comparable numbers of islets with good separation of exocrine and islet tissue after a similar period of digestion. There were no differences in the number, size, purity, or viability of islets between the two groups. In conclusion, UW solution offers no benefits over HOC for pancreata with short cold ischemic times, and because of its expense and need to use greater amounts of collagenase enzyme, we continue to use HOC.
G S Robertson; D Chadwick; S Thirdborough; S Swift; J Davies; R James; P R Bell; N J London
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Publication Detail:
Type:  Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation     Volume:  56     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-10-28     Completed Date:  1993-10-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  550-3     Citation Subset:  IM    
Department of Surgery, Leicester Royal Infirmary, United Kingdom.
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MeSH Terms
Cell Survival
Citrates / chemistry
Citric Acid
Cold Temperature / adverse effects
Collagenases / metabolism
Ischemia / etiology
Islets of Langerhans* / cytology
Organ Preservation Solutions*
Osmolar Concentration
Pancreas / blood supply*
Prospective Studies
Random Allocation
Tissue Donors
Reg. No./Substance:
0/Citrates; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 512-69-6/Raffinose; 58-61-7/Adenosine; 70-18-8/Glutathione; 77-92-9/Citric Acid; EC 3.4.24.-/Collagenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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