Document Detail

Human intravenous immunoglobulins suppress seizure activities and inhibit the activation of GFAP-positive astrocytes in the hippocampus of picrotoxin- kindled rats.
MedLine Citation:
PMID:  22082354     Owner:  NLM     Status:  Publisher    
Abstract Objective: We previously showed that human intravenous immunoglobulin (IVIG) can lower seizure severity and prolong seizure latency in picrotoxin-kindled rats. The aim of this study was to further characterize the effects of IVIG on seizure activity and investigate its influence on astrocytes in the hippocampus of picrotoxin-kindled rats. Methods: A rat kindling model was established by peritoneal injections of picrotoxin for 21 days in Wistar rats. Seventy-five rats were equally divided into 5 groups: picrotoxin, IVIG pretreatment, IVIG post-treatment, normal saline control, IVIG control. Seizure severity was evaluated according to a 6-stage classification. The number and morphology of glial fibrillary acidic protein (GFAP)-positive astrocytes was studied by immunohistochemistry using the anti-GFAP antibody. The cross-sectional area and grayscale of GFAP-positive astrocytes were also determined. Results: In picrotoxin-kindled rats, pretreatment with IVIG appeared to inhibit full kindling rates and it significantly reduced the number of GFAP-positive cells in the hippocampus (P<0.001). IVIG also significantly (P<0.001) attenuated the increase in the cross-sectional area and grayscale of GFAP-positive astrocytes in the hippocampus. Conclusion: Our results suggest that by suppressing the expression of GFAP, IVIGs may reduce seizure activity and inhibit the activation of GFAP-positive astrocytes in picrotoxin-kindled rats.
Dongfang Li; Pinggan Li; Zhanwen He; Danyang Cen; Zhe Meng; Liyang Liang; Xiangyang Luo
Related Documents :
21993474 - Variation in young's modulus along the length of a rat vibrissa.
22002084 - Derivation of rat embryonic stem cells and generation of protease-activated receptor-2 ...
6145494 - A kinetic scattering approach to the non-linear instabilities of rat brain tyrosine hyd...
16115684 - Relationship of the predatory attack experience to neural plasticity, pcreb expression ...
2177764 - Steroid fatty acid esters in adrenals and plasma: effects of acth.
15135004 - Biologic feature of prostatic hyperplasia developed in spontaneously hypertensive rats.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-14
Journal Detail:
Title:  The International journal of neuroscience     Volume:  -     ISSN:  1563-5279     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0270707     Medline TA:  Int J Neurosci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1Department of Children's Neuro-endocrinology, the Memorial Hospital of Sun Yat-sen University , Guangzhou 510120 , China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Altered physiology of acid secretion in depression-prone Flinders rats results in exacerbated NSAID ...
Next Document:  Are there independent predisposing factors for postoperative infections following open heart surgery...