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Human intravenous immunoglobulins suppress seizure activities and inhibit the activation of GFAP-positive astrocytes in the hippocampus of picrotoxin- kindled rats.
MedLine Citation:
PMID:  22082354     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Objective: We previously showed that human intravenous immunoglobulin (IVIG) can lower seizure severity and prolong seizure latency in picrotoxin-kindled rats. The aim of this study was to further characterize the effects of IVIG on seizure activity and investigate its influence on astrocytes in the hippocampus of picrotoxin-kindled rats. Methods: A rat kindling model was established by peritoneal injections of picrotoxin for 21 days in Wistar rats. Seventy-five rats were equally divided into 5 groups: picrotoxin, IVIG pretreatment, IVIG post-treatment, normal saline control, IVIG control. Seizure severity was evaluated according to a 6-stage classification. The number and morphology of glial fibrillary acidic protein (GFAP)-positive astrocytes was studied by immunohistochemistry using the anti-GFAP antibody. The cross-sectional area and grayscale of GFAP-positive astrocytes were also determined. Results: In picrotoxin-kindled rats, pretreatment with IVIG appeared to inhibit full kindling rates and it significantly reduced the number of GFAP-positive cells in the hippocampus (P<0.001). IVIG also significantly (P<0.001) attenuated the increase in the cross-sectional area and grayscale of GFAP-positive astrocytes in the hippocampus. Conclusion: Our results suggest that by suppressing the expression of GFAP, IVIGs may reduce seizure activity and inhibit the activation of GFAP-positive astrocytes in picrotoxin-kindled rats.
Authors:
Dongfang Li; Pinggan Li; Zhanwen He; Danyang Cen; Zhe Meng; Liyang Liang; Xiangyang Luo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-14
Journal Detail:
Title:  The International journal of neuroscience     Volume:  -     ISSN:  1563-5279     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0270707     Medline TA:  Int J Neurosci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Department of Children's Neuro-endocrinology, the Memorial Hospital of Sun Yat-sen University , Guangzhou 510120 , China.
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