Document Detail


Human intestinal epithelial cells are susceptible to influenza virus subtype H9N2.
MedLine Citation:
PMID:  21986059     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Avian influenza viruses (AIV) replicate efficiently in guts of birds, and virus shedding is critical to viral transmission among birds and from birds to other species. In this study, we showed that an H9N2 viral strain, isolated from a human patient, caused typical influenza-like signs and illness including loss of body weight in Balb/c mice, and that viral RNA could be detected in intestinal tissues. We demonstrated that human intestinal epithelial cell line HT-29 was susceptible to the virus, and the infected cells went apoptotic at the early stage post infection. Compared to a pandemic (H1N1) 2009 influenza isolate, we found that the human H9N2 virus induced more severe apoptotic and stronger innate immune responses. Both extrinsic and intrinsic apoptotic pathways were activated in human intestinal epithelial cells, and the levels of FasL and TNF-α were induced up to hundreds-fold in response to the H9N2 infection. Interestingly, Bcl-2 family member Bid was cleaved during the cause of infection, and the truncated Bid (tBid) appeared to play a role in the initiation of the intrinsic apoptosis with increased release of cytochrome c in cytosol. As for pro-inflammatory responses in H9N2-infected intestinal epithelial cells, RANTES and IP10 were induced significantly and may have played a major role in intestinal pathogenicity. Moreover, TLR-8, MyD88, and MDA-5 were all up-regulated in the infection, critical in the induction of IFN-β and host innate immunity against the H9N2 virus. Our findings have demonstrated a unique pattern of host responses in human gut in response to H9N2 subtype influenza viruses, which will broaden our understanding of the pathogenesis of AIV infection in both human and animals.
Authors:
Bingqian Qu; Xue Li; Wei Gao; Wenkui Sun; Yu Jin; Carol J Cardona; Zheng Xing
Related Documents :
1708159 - Cytomegalovirus-antibody-negative lymphocytes have increased permissiveness for human i...
16537639 - Functional central polypurine tract provides downstream protection of the human immunod...
1540099 - A national survey of surgeons' attitudes about patients with human immunodeficiency vir...
2910209 - Failure of erythromycin to cure secondary syphilis in a patient infected with the human...
3332779 - Epidemiology and control of sexually transmitted diseases: strategic evolution.
2337949 - Inhibition of replication of human immunodeficiency virus by a heteropolyoxotungstate (...
2161559 - Homology of cytokine synthesis inhibitory factor (il-10) to the epstein-barr virus gene...
204579 - Reactivation of latent herpes simplex virus infection of the autonomic nervous system b...
5953019 - Quantitative studies on fabrics as disseminators of viruses. i. persistence of vaccinia...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-22
Journal Detail:
Title:  Virus research     Volume:  -     ISSN:  1872-7492     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-10-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
The Key Laboratory of Pharmaceutical Biotechnology and Medical School, Nanjing University, Nanjing, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Diffusion-Weighted MRI Helps Predict Outcome in Basilar Artery Occlusion Patients Treated with Intra...
Next Document:  Decreased Expression of EBI3 and Foxp3 in CD4+CD25+ Regulatory T Cells in Murine Experimental Allerg...