| Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. | |
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MedLine Citation:
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PMID: 8642659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vpr is a 96-amino-acid protein encoded by human immunodeficiency virus type 1 (HIV-1) that prevents proliferation of infected cells. We have established a system for infection of 100% of a T-cell population with HIV and use this system to show that within the context of HIV-1 infection, Vpr is primarily cytostatic rather than cytotoxic. Vpr acts upstream of dephosphorylation of the mitotic cyclin-dependent kinase, and causes infected cells to accumulate in the G2 stage of the cell cycle. However, some HIV-1 infected cells increase in ploidy and size, accumulating DNA to an 8N level. Furthermore, the mechanism of the Vpr mitotic block is qualitatively different from that of G2 DNA damage checkpoint control. |
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Authors:
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S R Bartz; M E Rogel; M Emerman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 70 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-07-18 Completed Date: 1996-07-18 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2324-31 Citation Subset: IM; X |
Affiliation:
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Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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CDC2 Protein Kinase
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metabolism Cell Cycle* Cell Line Cyclins / metabolism DNA Damage G2 Phase* Gene Products, vpr / physiology* HIV-1 / pathogenicity, physiology* Hela Cells Humans Polyploidy T-Lymphocytes / cytology, virology vpr Gene Products, Human Immunodeficiency Virus |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI30927/AI/NIAID NIH HHS; T32-CA09229/CA/NCI NIH HHS; UI AI-93-021/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cyclins; 0/Gene Products, vpr; 0/vpr Gene Products, Human Immunodeficiency Virus; EC 2.7.11.22/CDC2 Protein Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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