Document Detail

Human immunodeficiency virus type-1 vulnerates nascent neuronal cells.
MedLine Citation:
PMID:  18380805     Owner:  NLM     Status:  MEDLINE    
Macrophages or microglial cells are the major target cells for HIV-1 infection in the brain. The infected cells release neurotoxic factors that may cause severe neuronal cell damage, especially in the basal ganglia and hippocampus. In this study, we used rat OHC to examine the region-specific neuronal cell damage caused by HIV-1-infected macrophages. When OHC was cocultured with HIV-1-infected MDM, we found that neuronal cells at the GCL of the DG were preferentially killed via apoptosis, and that projection of MF from GCL to PCL of the CA3 region was severely disturbed. We marked precursor cells around the DG region by using an EGFP-expressing retrovirus vector and found that these cells lost the ability to differentiate into neurons when exposed to HIV-1-infected MDM. In the DG, new neurons are normally incorporated into GCL or PCL, while in the presence of HIV-1-infected MDM, mature neurons failed to be incorporated into those layers. These data indicate that the neurotoxic factor(s) released from HIV-1-infected macrophages impede(s) neuronal cell repair in brain tissue. This suggests that DG is the region of the hippocampus most vulnerable to neuronal damage caused by HIV-1 infection, and that its selective vulnerability is most likely due to the highly active neurogenesis that takes place in this region.
Hiroko Kitayama; Yoshiharu Miura; Yoshinori Ando; Yoshio Koyanagi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology and immunology     Volume:  52     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-04-02     Completed Date:  2008-09-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  78-88     Citation Subset:  IM    
Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto, Japan.
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MeSH Terms
Brain / pathology*
Coculture Techniques
HIV-1 / physiology*
Macrophages / virology*
Models, Biological
Neurons / pathology*
Organ Culture Techniques

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