| Human immunodeficiency virus type 1 reverse-transcriptase and protease subtypes: classification, amino acid mutation patterns, and prevalence in a northern California clinic-based population. | |
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MedLine Citation:
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PMID: 11574914 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phylogenetic analysis of the reverse transcriptase (RT) and protease of 117 published complete human immunodeficiency virus (HIV) type 1 genome sequences demonstrated that these genes cluster into distinct subtypes. There was a slightly higher proportion of informative sites in the RT (40.4%) than in the protease (34.8%; P= .03). Although most variation between subtypes was due to synonymous nucleotide substitutions, several subtype-specific amino acid patterns were observed. In the protease, the subtype-specific variants included 7 positions associated with drug resistance. Variants at positions 10, 20, 36, and 82 were more common in non-B isolates, whereas variants at positions 63, 77, and 93 were more common in subtype B isolates. In the RT, the subtype-specific mutations did not include positions associated with anti-retroviral drug resistance. RT and protease sequences from 2246 HIV-infected persons in northern California were also examined: 99.4% of the sequences clustered with subtype B, whereas 0.6% clustered with subtype A, C, or D. |
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Authors:
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M J Gonzales; R N Machekano; R W Shafer |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2001-09-10 |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 184 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-09-27 Completed Date: 2001-12-07 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 998-1006 Citation Subset: AIM; IM |
Affiliation:
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Division of Infectious Diseases, Stanford University Medical Center, Stanford, California 94305, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Genome, Viral HIV Protease / genetics* HIV Reverse Transcriptase / genetics* HIV-1 / genetics* Humans Mutation* North Carolina Phylogeny Sequence Alignment Sequence Homology, Amino Acid |
| Grant Support | |
ID/Acronym/Agency:
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AI-46148/AI/NIAID NIH HHS; R01 AI046148-08/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.49/HIV Reverse Transcriptase; EC 3.4.23.-/HIV Protease |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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