Document Detail


Human immunodeficiency virus envelope protein Gp120 induces proliferation but not apoptosis in osteoblasts at physiologic concentrations.
MedLine Citation:
PMID:  21931863     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Patients with HIV infection have decreased numbers of osteoblasts, decreased bone mineral density and increased risk of fracture compared to uninfected patients; however, the molecular mechanisms behind these associations remain unclear. We questioned whether Gp120, a component of the envelope protein of HIV capable of inducing apoptosis in many cell types, is able to induce cell death in bone-forming osteoblasts. We show that treatment of immortalized osteoblast-like cells and primary human osteoblasts with exogenous Gp120 in vitro at physiologic concentrations does not result in apoptosis. Instead, in the osteoblast-like U2OS cell line, cells expressing CXCR4, a receptor for Gp120, had increased proliferation when treated with Gp120 compared to control (P<0.05), which was inhibited by pretreatment with a CXCR4 inhibitor and a G-protein inhibitor. This suggests that Gp120 is not an inducer of apoptosis in human osteoblasts and likely does not directly contribute to osteoporosis in infected patients by this mechanism.
Authors:
Nathan W Cummins; Anna Klicpera; Amy M Sainski; Gary D Bren; Sundeep Khosla; Jennifer J Westendorf; Andrew D Badley
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-09-12
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-20     Completed Date:  2012-02-28     Revised Date:  2014-04-23    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e24876     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*,  genetics
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
HIV Envelope Protein gp120 / pharmacology*
Humans
Ki-67 Antigen / genetics,  metabolism
Osteoblasts / cytology*,  drug effects*,  metabolism
Receptors, CXCR4 / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
AI 40384/AI/NIAID NIH HHS; AR 056950-02/AR/NIAMS NIH HHS; T32 AR056950/AR/NIAMS NIH HHS; T32 GM072474/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/CXCR4 protein, human; 0/HIV Envelope Protein gp120; 0/Ki-67 Antigen; 0/Receptors, CXCR4
Comments/Corrections

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