Document Detail

Human immature dental pulp stem cells' contribution to developing mouse embryos: production of human/mouse preterm chimaeras.
MedLine Citation:
PMID:  19236382     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: In this study, we aimed at determining whether human immature dental pulp stem cells (hIDPSC) would be able to contribute to different cell types in mouse blastocysts without damaging them. Also, we analysed whether these blastocysts would progress further into embryogenesis when implanted to the uterus of foster mice, and develop human/mouse chimaera with retention of hIDPSC derivates and their differentiation. MATERIALS AND METHODS: hIDPSC and mouse blastocysts were used in this study. Fluorescence staining of hIDPSC and injection into mouse blastocysts, was performed. Histology, immunohistochemistry, fluorescence in situ hybridization and confocal microscopy were carried out. RESULTS AND CONCLUSION: hIDPSC showed biological compatibility with the mouse host environment and could survive, proliferate and contribute to the inner cell mass as well as to the trophoblast cell layer after introduction into early mouse embryos (n = 28), which achieved the hatching stage following 24 and 48 h in culture. When transferred to foster mice (n = 5), these blastocysts with hIDPSC (n = 57) yielded embryos (n = 3) and foetuses (n = 6); demonstrating presence of human cells in various organs, such as brain, liver, intestine and hearts, of the human/mouse chimaeras. We verified whether hIDPSC would also be able to differentiate into specific cell types in the mouse environment. Contribution of hIDPSC in at least two types of tissues (muscles and epithelial), was confirmed. We showed that hIDPSC survived, proliferated and differentiated in mouse developing blastocysts and were capable of producing human/mouse chimaeras.
S A Siqueira da Fonseca; S Abdelmassih; T de Mello Cintra Lavagnolli; R C Serafim; E J Clemente Santos; C Mota Mendes; V de Souza Pereira; C E Ambrosio; M A Miglino; J A Visintin; R Abdelmassih; A Kerkis; I Kerkis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-24
Journal Detail:
Title:  Cell proliferation     Volume:  42     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-25     Completed Date:  2009-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  132-40     Citation Subset:  IM    
Stem Cell Laboratory, Roger Abdelmassih Human Reproduction Clinic and Research Center, Sao Paulo, Brazil.
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MeSH Terms
Adult Stem Cells / cytology*,  transplantation
Animal Structures / cytology,  embryology,  metabolism
Blastocyst / cytology
Cell Differentiation / physiology
Chromosomes, Human, Y / chemistry
Dental Pulp / cytology*
Embryo Transfer
Embryo, Mammalian / cytology*,  embryology
Embryonic Development / physiology*
Epithelial Cells / cytology,  metabolism
Epithelium / embryology,  metabolism
Fetus / cytology*,  embryology,  metabolism
In Situ Hybridization, Fluorescence
Mice, Inbred Strains
Muscle Cells / cytology,  metabolism
Muscles / cytology,  embryology,  metabolism
Transplantation Chimera / embryology*,  metabolism

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