| Human homologue of SETA binding protein 1 interacts with cathepsin B and participates in TNF-Induced apoptosis in ovarian cancer cells. | |
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MedLine Citation:
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PMID: 16733801 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lysosomal cysteine protease cathepsin B has been reported to play an important role in apoptosis of many different cancer cells, but the regulation of cathepsin B in apoptosis is poorly understood. Human homologue of SETA binding protein 1 (hSB1) was identified to interact with cathepsin B by yeast-two hybrid method, and the interaction was confirmed in vitro GST pull-down assay and in vivo coimmunoprecipitation experiment. hSB1 was co-localized with cathepsin B in cellular lysosomes. Our previous study has shown that TNF can induce ovarian cancer cells OV-90 apoptosis and the apoptosis process is cathepsin B-depended. Here we provide evidence that overexpression of cathepsin B-interacting protein hSB1 could suppress TNF-triggered apoptosis in OV-90 cells, but has no effect on cellular cathepsin B activity. hSB1 may function as a regulator of cathepsin B-mediated apoptosis. |
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Authors:
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Jian-Ping Liu; Nan-Song Liu; Han-Ying Yuan; Qian Guo; Hong Lu; Yu-Yang Li |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 292 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-03 Completed Date: 2007-01-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 189-95 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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metabolism* Apoptosis / drug effects* Cathepsin B / metabolism* Female Gene Expression / drug effects Gene Expression Profiling Humans Intracellular Signaling Peptides and Proteins / metabolism* Lysosomes / drug effects Ovarian Neoplasms / pathology* Protein Binding / drug effects Protein Transport / drug effects Reproducibility of Results Sequence Homology* Tumor Necrosis Factor-alpha / pharmacology* Two-Hybrid System Techniques |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Intracellular Signaling Peptides and Proteins; 0/SETA binding protein 1, human; 0/Tumor Necrosis Factor-alpha; EC 3.4.22.1/Cathepsin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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