Document Detail


Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.
MedLine Citation:
PMID:  2195549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Entry into mitosis during the somatic cell cycle is regulated in response to signals that monitor the completion of DNA replication, the integrity of the nuclear genome, and, possibly, the increase in cellular mass during the cell cycle. It has been postulated that the operation of this cell cycle control involves the gradual accumulation of rate-limiting mitotic inducers, which trigger nuclear division when their cellular concentration reaches a critical level. We have cloned a human gene, which we call CDC25, whose product may function as a mitotic inducer. This human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25+ mitotic inducer. The human CDC25 gene rescues the defect of a fission yeast temperature-sensitive (ts) cdc25ts mutant that is unable to initiate mitosis. In HeLa cells CDC25 mRNA levels are very low in G1 and increase at least 4-fold as cells progress towards M phase. These data suggest that in human cells, as in fission yeast, the accumulation of CDC25 mitotic inducer during G2 may play a key role in regulating the timing of mitosis.
Authors:
K Sadhu; S I Reed; H Richardson; P Russell
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  87     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-08-15     Completed Date:  1990-08-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5139-43     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M34065
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cloning, Molecular / methods
Gene Expression
Gene Library
Hela Cells / cytology
Humans
Interphase*
Kinetics
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Proteins / genetics*
RNA, Messenger / genetics
Schizosaccharomyces / genetics*
Sequence Homology, Nucleic Acid
cdc25 Phosphatases
Grant Support
ID/Acronym/Agency:
GM38328/GM/NIGMS NIH HHS; GM41281/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Oligonucleotide Probes; 0/Proteins; 0/RNA, Messenger; EC 3.1.3.48/cdc25 Phosphatases
Comments/Corrections

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