| Human herpesvirus type 8 variants circulating in Europe, Africa and North America in classic, endemic and epidemic Kaposi's sarcoma lesions during pre-AIDS and AIDS era. | |
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MedLine Citation:
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PMID: 20079510 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatrogenic, endemic as well as epidemic Kaposi's sarcoma (KS) during pre-AIDS and AIDS period (1971-2008) in countries with different KS incidence rate. DNA samples from cutaneous KS lesions of 68 patients living in Africa (n=23, Cameroon, Kenya and Uganda), Europe (n=34, Greece and Italy) and North America (n=11) have been subjected to PCR amplification of HHV-8 ORF 26, T0.7, K1 and K14.1/15, followed by direct nucleotide sequencing and phylogenetic analysis. Among the 23 African samples, the majority of HHV-8 ORF 26 variants clustered with the subtype R (n=12) and B (n=5). Conversely, the viral sequences obtained from 45 European and North European tumors belonged mainly to subtype A/C (n=36). In general, HHV-8 and K1 variant clustering paralleled that of ORF 26 and T0.7. Genotyping of the K14.1/15 loci revealed a large predominance of P subtype in all tumors. In conclusion, comparison of the HHV-8 sequences from classic or endemic versus AIDS-associated KS showed a strong linkage of the HHV-8 variants with specific populations, which has not changed during AIDS epidemic. |
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Authors:
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Maria Lina Tornesello; Benon Biryahwaho; Robert Downing; Angelo Hatzakis; Elvio Alessi; Marco Cusini; Vincenzo Ruocco; Edward Katongole-Mbidde; Giovanna Loquercio; Luigi Buonaguro; Franco M Buonaguro |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-15 |
Journal Detail:
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Title: Virology Volume: 398 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-03-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 280-9 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Molecular Biology and Viral Oncology and AIDS Reference Centre, National Cancer Institute "Fondazione Pascale", Cappella Cangiani, 80131 Naples, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acquired Immunodeficiency Syndrome
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complications,
epidemiology,
virology* Adult Africa / epidemiology Aged Europe / epidemiology Female Genes, Viral / genetics Genetic Variation / genetics Genotype Herpesviridae Infections / epidemiology, virology* Herpesvirus 8, Human / genetics* Humans Male Middle Aged North America / epidemiology Point Mutation / genetics Polymerase Chain Reaction Polymorphism, Genetic / genetics Sarcoma, Kaposi / epidemiology, virology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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