| Human herpesvirus 6B inhibits cell proliferation by a p53-independent pathway. | |
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MedLine Citation:
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PMID: 17276372 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Various forms of cellular stress can activate the tumour suppressor protein p53, an important regulator of cell cycle arrest, apoptosis, and cellular senescence. Cells infected by human herpesvirus 6B (HHV-6B) accumulate aberrant amounts of p53. OBJECTIVES: The aim of this study was to investigate the role of p53 accumulation in the HHV-6B-induced cell cycle arrest. STUDY DESIGN: The role of p53 was studied using the p53 inhibitor pifithrin-a, and cells genetically deficient in functional p53 by homologous recombination. RESULTS: In response to HHV-6B infection, epithelial cells were arrested in the G1/S phase of the cell cycle concomitant with an aberrant accumulation of p53. However, the known p53-induced mediator of cell cycle arrest, p21, was not upregulated. Approximately 90% of the cells expressed HHV-6B p41, indicative of viral infection. The presence of pifithrin-a, a p53 inhibitor, did not reverse the HHV-6B-induced cell cycle block. In support of this, HHV-6B infection of p53(-/-) cells induced a cell cycle block before S-phase with kinetics similar to or faster than that observed by infection in wt cells. CONCLUSIONS: HHV-6B infection inhibited host cell proliferation concomitantly with p53 accumulation, but importantly the block in cell cycle occurred by a pathway independent of p53. |
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Authors:
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Bodil Øster; Maja D Kaspersen; Emil Kofod-Olsen; Bettina Bundgaard; Per Höllsberg |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Volume: 37 Suppl 1 ISSN: 1386-6532 ISO Abbreviation: J. Clin. Virol. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2007-02-05 Completed Date: 2007-08-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815671 Medline TA: J Clin Virol Country: Netherlands |
Other Details:
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Languages: eng Pagination: S63-8 Citation Subset: IM |
Affiliation:
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Institute of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Benzothiazoles
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pharmacology Cell Cycle Cell Line Cell Proliferation* Cyclin-Dependent Kinase Inhibitor p21 / biosynthesis DNA-Binding Proteins / biosynthesis Herpesvirus 6, Human / physiology* Humans Toluene / analogs & derivatives, pharmacology Tumor Suppressor Protein p53 / antagonists & inhibitors, biosynthesis, physiology* Viral Proteins / biosynthesis Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/Benzothiazoles; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/DNA-Binding Proteins; 0/Tumor Suppressor Protein p53; 0/Viral Proteins; 0/pifithrin; 108-88-3/Toluene; 138415-18-6/p41 protein, Human herpesvirus 6 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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