| Human hepatoma (Hep G2) cultures contain salt-resistant triglyceridase ("liver lipase"). | |
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MedLine Citation:
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PMID: 2419721 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The culture fluid of Hep G2 human hepatoma cells contains triglyceridase activity resistant to high-salt concentrations. The lipase binds to Sepharose-heparin columns from which it can be eluted by 0.8 to 0.9 M NaCl. The nature of this lipase was studied using antibodies raised against "liver" lipases from human and rat origin. The anti-rat liver lipase inhibits both the postheparin human and rat plasma enzyme while the anti-human liver lipase has no effect on the rat enzyme. The lipase of the Hep G2 cultures showed affinity to the antibodies raised against rat as well as human "liver" lipase as shown by inhibition experiments. These results show that Hep G2 cells secrete "liver" lipase and that there seems to exist a structural homology between the lipases from rat and human origin. |
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Authors:
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N L Persoon; H J Sips; H Jansen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Life sciences Volume: 38 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 1986 Mar |
Date Detail:
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Created Date: 1986-04-21 Completed Date: 1986-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1029-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology Carcinoma, Hepatocellular / enzymology* Cell Line Chromatography, Affinity Epitopes / immunology Humans Lipase / immunology, metabolism* Liver Neoplasms / enzymology* Osmolar Concentration Rats Sodium Chloride / pharmacology Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Epitopes; 7647-14-5/Sodium Chloride; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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