Document Detail


Human growth hormone induces and maintains c-myc gene expression in Nb2 lymphoma cells.
MedLine Citation:
PMID:  4065045     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of human GH (hGH) on c-myc gene expression and cell proliferation in the Nb2 lymphoma cell line was examined. The addition of hormone to stationary cultures of Nb2 cells resulted in an increased accumulation of c-myc transcripts that was detectable within 15 min, reached a maximum induction of 25-fold in 3 h, and then gradually declined. Although maximal accumulation of c-myc transcripts occurred within 3 h after hGH addition, removal of the hormone even after 4 h of treatment resulted in a failure of the cells to proliferate. This loss of proliferative capacity following hormone removal was accompanied by a 20-fold reduction in the level of c-myc transcripts. These results indicate that hGH induced proliferation of Nb2 cells is associated with the induction and maintenance of c-myc gene expression.
Authors:
W H Fleming; P R Murphy; L J Murphy; T W Hatton; R J Matusik; H G Friesen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  117     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1985-12-27     Completed Date:  1985-12-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2547-9     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Cell Line
Gene Expression Regulation / drug effects*
Growth Hormone / pharmacology*
Humans
Lymphoma / genetics*
Oncogenes*
RNA, Messenger / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
HDO7843-13/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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