| Human globin chain separation by capillary electrophoresis in acidic isoelectric buffers. | |
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MedLine Citation:
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PMID: 9719553 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and reliable method, utilizing capillary electrophoresis in uncoated capillaries in acidic isoelectric buffers, is reported for screening for thalassemia and other defects on the synthesis of human globin chains. A solution of 50 mM iminodiacetic acid (pI 2.23), containing 7 M urea and 0.5% hydroxyethylcellulose (apparent pH 3.2) is used as background electrolyte for fast separation of heme-free, denatured globin (alpha, beta and gamma) chains. Due to the low conductivity of such a buffer, high voltage gradients (600 V/cm) can be applied, thus reducing the separation time to only a few minutes. It is additionally shown that inclusion of 2% surfactant (Tween 20) in the background electrolyte induces the splitting of the gamma chains into two zones, called Agamma and Ggamma, which represent the products of two genes coding for Ala or Gly as residue 136 of the chain. |
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Authors:
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P G Righetti; A Saccomani; A V Stoyanov; C Gelfi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 19 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-11-09 Completed Date: 1998-11-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: GERMANY |
Other Details:
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Languages: eng Pagination: 1733-7 Citation Subset: IM |
Affiliation:
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Department of Agricultural and Industrial Biotechnology, University of Verona, Italy. righetti@imiucca.csi.unimi.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acids Adult Buffers Electrophoresis, Capillary / methods* Globins / analysis* Humans |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Buffers; 9004-22-2/Globins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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