Document Detail


Human gamma X satellite DNA: an X chromosome specific centromeric DNA sequence.
MedLine Citation:
PMID:  8585987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cosmid clone, CX16-2D12, was previously localized to the centromeric region of the human X chromosome and shown to lack human X-specific alpha satellite DNA. A 1.2 kb EcoRI fragment was subcloned from the CX16-2D12 cosmid and was named 2D12/E2. DNA sequencing revealed that this 1,205 bp fragment consisted of approximately five tandemly repeated DNA monomers of 220 bp. DNA sequence homology between the monomers of 2D12/E2 ranged from 72.8% to 78.6%. Interestingly, DNA sequence analysis of the 2D12/E2 clone displayed a change in monomer unit orientation between nucleotide positions 585-586 from a "tail-to-head" arrangement to a "head-to-tail" configuration. This may reflect the existence of at least one inversion within this repetitive DNA array in the centromeric region of the human X chromosome. The DNA consensus sequence derived from a compilation of these 220 bp monomers had approximately 62% DNA sequence similarity to the previously determined gamma 8 satellite DNA consensus sequence. Comparison of the 2D12/E2 and gamma 8 consensus sequences revealed a 20 bp DNA sequence that was well conserved in both DNA consensus sequences. Slot-blot analysis revealed that this repetitive DNA sequence comprises approximately 0.015% of the human genome, similar to that found with gamma 8 satellite DNA. These observations suggest that this satellite DNA clone is derived from a subfamily of gamma satellite DNA and is thus designated gamma X satellite DNA. When genomic DNA from six unrelated males and two unrelated females was cut with SstI or HpaI and separated by pulsed-field gel electrophoresis, no restriction fragment length polymorphisms were observed for either gamma X (2D12/E2) or gamma 8 (50E4) probes. Fluorescence in situ hybridization localized the 2D12/E2 clone to the lateral sides of the primary constriction specifically on the human X chromosome.
Authors:
C Lee; X Li; E W Jabs; D Court; C C Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  104     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-03-28     Completed Date:  1996-03-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  103-12     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine and Pathology, University of Alberta and the University of Alberta Hospitals, Edmonton, Alberta, Canada, T6G 2B7.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X87951
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Centromere / genetics*
Chromosome Mapping
Cloning, Molecular
Conserved Sequence*
DNA, Satellite / chemistry*,  genetics,  isolation & purification
Female
Gene Dosage
Humans
In Situ Hybridization, Fluorescence
Male
Molecular Sequence Data
Restriction Mapping
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
X Chromosome*
Grant Support
ID/Acronym/Agency:
HD22819/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Satellite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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