| Human fibroblast commitment to a senescence-like state in response to histone deacetylase inhibitors is cell cycle dependent. | |
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MedLine Citation:
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PMID: 8756678 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human diploid fibroblasts (HDF) complete a limited number of cell divisions before entering a growth arrest state that is termed replicative senescence. Two histone deacetylase inhibitors, sodium butyrate and trichostatin A, dramatically reduce the HDF proliferative life span in a manner that is dependent on one or more cell doublings in the presence of these agents. Cells arrested and subsequently released from histone deacetylase inhibitors display markers of senescence and exhibit a persistent G1 block but remain competent to initiate a round of DNA synthesis in response to simian virus 40 T antigen. Average telomere length in prematurely arrested cells is greater than in senescent cells, reflecting a lower number of population doublings completed by the former. Taken together, these results support the view that one component of HDF senescence mimics a cell cycle-dependent drift in differentiation state and that propagation of HDF in histone deacetylase inhibitors accentuates this component. |
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Authors:
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V V Ogryzko; T H Hirai; V R Russanova; D A Barbie; B H Howard |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular biology Volume: 16 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1996 Sep |
Date Detail:
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Created Date: 1996-09-26 Completed Date: 1996-09-26 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5210-8 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892-2753, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Viral, Tumor
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genetics,
physiology Butyric Acid Butyric Acids / pharmacology* Cell Aging / drug effects*, physiology Cell Cycle / physiology* Cell Division Cell Line Enzyme Inhibitors / pharmacology* Fibroblasts / chemistry, cytology, drug effects* Hela Cells / drug effects Histone Deacetylase Inhibitors* Histone Deacetylases / physiology Humans Hydroxamic Acids / pharmacology* Retinoblastoma Protein / physiology Telomere / ultrastructure Transfection |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral, Tumor; 0/Butyric Acids; 0/Enzyme Inhibitors; 0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 0/Retinoblastoma Protein; 107-92-6/Butyric Acid; 58880-19-6/trichostatin A; EC 3.5.1.98/Histone Deacetylases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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