Document Detail


Human erythroleukemic (HEL) cells express a platelet P2T-like ADP receptor.
MedLine Citation:
PMID:  7740516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment of Fura-2 loaded HEL cells, a human megakaryocyte-like cell line, with P2-purinoceptor nucleotide ligands (ADP, ATP, UTP, 2-methylthio-ATP) evoked a rise in cytosolic calcium. Homologous- and cross-desensitization studies using sequential addition of nucleotides showed that the ADP-induced calcium response in HEL cells was mediated mainly by purinoceptor(s) for which ADP but not ATP was an agonist. There were also minor contributions from purinoceptors for which ATP and ADP are both agonists (probably P2U and P2Y). ATP inhibited the ADP-induced calcium response in HEL cells. This inhibition was overcome by raising the ADP concentration, thus indicating that ATP was an antagonist for the HEL cell ADP receptor. AMP was also an antagonist, albeit weak, for the HEL cell ADP receptor. Antagonism of the ADP-induced calcium response by ATP was similarly observed in MEG-01 cells, another human megakaryocyte-like cell line, but not in 293 cells, a nonhematopoietic cell line. These studies suggest that HEL cells express an ADP receptor for which ATP and AMP are antagonists. This characteristic of the HEL cell ADP receptor is also displayed by the platelet P2T receptor. Thus, HEL cells appear to be a surrogate source of the platelet ADP receptor.
Authors:
X P Shi; K C Yin; S J Gardell
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  77     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-06-05     Completed Date:  1995-06-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  235-47     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, Merck Research Laboratories, West Point, PA 19486, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism,  pharmacology*
Adenosine Monophosphate / pharmacology
Adenosine Triphosphate / analogs & derivatives,  pharmacology
Biological Transport / drug effects
Blood Platelets / chemistry
Calcium / metabolism
Humans
Leukemia, Erythroblastic, Acute / metabolism,  pathology*
Megakaryocytes / metabolism*
Neoplasm Proteins / metabolism*
Neoplastic Stem Cells / metabolism*
Platelet Activation
Receptors, Purinergic P2 / antagonists & inhibitors,  classification,  metabolism*
Thionucleotides / pharmacology
Tumor Cells, Cultured
Uridine Triphosphate / pharmacology
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Receptors, Purinergic P2; 0/Thionucleotides; 43170-89-4/2-methylthio-ATP; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 61-19-8/Adenosine Monophosphate; 63-39-8/Uridine Triphosphate; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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