Document Detail

Human erythrocytes inhibit complement-mediated solubilization of immune complexes.
MedLine Citation:
PMID:  2522967     Owner:  NLM     Status:  MEDLINE    
Incubation of precipitable immune complexes (IC) with fresh human serum or guinea pig serum resulted in solubilization of IC. When packed human E were added to human serum or guinea pig serum, binding of IC to the E occurred and IC solubilization was significantly inhibited. By contrast, SRBC did not bind IC nor inhibit IC solubilization. Because IC binding to human E is mediated by CR type 1 (CR1) we evaluated whether CR1 was responsible for the inhibition of IC solubilization. Human E were treated with trypsin or anti-CR1 mAb. Both treatments abrogated IC binding to human E but did not affect the ability of the human E to inhibit IC solubilization. Human E inhibited C activation by IC. Thus, incubation of IC in human serum caused significant activation of C3 and C5, but not C4. However, when IC were incubated in whole blood or with isolated human E and serum, C3 activation by IC was inhibited significantly. In addition, we demonstrated that the C3b generated during C activation by IC deposited on both IC and human E. Thus, human E may compete for nascent C3 generated during C activation by IC. In conclusion, human E inhibit both complement-mediated solubilization of IC and C activation by IC.
B L Dorval; F G Cosio; D J Birmingham; L A Hebert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  142     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-22     Completed Date:  1989-05-22     Revised Date:  2013-08-23    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2721-7     Citation Subset:  AIM; IM    
Department of Internal Medicine, Ohio State University College of Medicine, Columbus 43210-1228.
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MeSH Terms
Antigen-Antibody Complex / metabolism*
Complement Activation
Complement C3 / analogs & derivatives,  analysis,  metabolism
Complement C3a* / analogs & derivatives*
Complement System Proteins / physiology*
Erythrocytes / physiology*
Receptors, Complement / physiology
Receptors, Complement 3b
Grant Support
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Complement C3; 0/Receptors, Complement; 0/Receptors, Complement 3b; 0/complement C3a, des-Arg-(77)-; 80295-42-7/Complement C3a; 9007-36-7/Complement System Proteins

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