| Human erythrocytes are not suitable for determination of intravascular volume of perfused rat liver. | |
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MedLine Citation:
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PMID: 16894404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Homologous or heterologous erythrocytes have been widely used for the estimation of intravascular volume of the liver. However, cross-species blood mediates immune response in the organ, and foreign cells are rapidly cleared from the plasma, indicating that heterologous erythrocytes may not be a suitable marker for determination of vascular space. This aspect was investigated in the perfused rat liver preparation following bolus administration of human (heterologous) erythrocytes into the portal vein. To compare the extent of its distribution within the liver, rat (homologous) erythrocytes and Evans blue were chosen as the reference vascular and extracellular markers, respectively. Hepatic distribution of human erythrocytes was influenced by the perfusion medium (with and without protein) and injection number (first and second injections). Mean transit time and hence volume of distribution decreased in the presence of protein and repetition of the injection. Even in the presence of protein, the volume of distribution obtained for human erythrocytes was larger than that of the extracellular volume of the liver obtained with Evans blue (0.22 +/- 0.01 vs. 0.20 +/- 0.02 ml/g), indicating that they are not suitable for determination of intravascular volume of the perfused rat liver preparation. |
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Authors:
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Y Karabey; S Sahin |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods and findings in experimental and clinical pharmacology Volume: 28 ISSN: 0379-0355 ISO Abbreviation: Methods Find Exp Clin Pharmacol Publication Date: 2006 Jul-Aug |
Date Detail:
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Created Date: 2006-08-08 Completed Date: 2006-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7909595 Medline TA: Methods Find Exp Clin Pharmacol Country: Spain |
Other Details:
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Languages: eng Pagination: 355-9 Citation Subset: IM |
Affiliation:
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Faculty of Pharmacy, Hacettepe University, Ankara, Turkey. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Blood Volume / physiology Blood Volume Determination / methods* Cell Survival Erythrocytes / physiology* Evans Blue Humans Kinetics Liver Circulation / physiology* Male Perfusion Rats Rats, Sprague-Dawley Serum Albumin, Bovine Tetrazolium Salts Thiazoles |
| Chemical | |
Reg. No./Substance:
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0/Serum Albumin, Bovine; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 314-13-6/Evans Blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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