Document Detail

Human endothelial cells produce orosomucoid, an important component of the capillary barrier.
MedLine Citation:
PMID:  9950854     Owner:  NLM     Status:  MEDLINE    
The serum protein orosomucoid (alpha1-acid glycoprotein) is needed to maintain the high capillary permselectivity required for normal homeostasis. It is not known how the protein executes its action, but it seems to contribute to the charge barrier. Moreover, recent studies suggest that the endothelial glycocalyx is essential for the charge barrier. The main site of orosomucoid synthesis is the liver, but we wanted to explore the possibility that orosomucoid was synthesized in endothelial cells. Primary cultures of human microvascular endothelial cells (HMVEC) from dermal tissue were established. Human liver cells were used as positive controls, and total RNA was prepared from both cell types. Reverse transcription-polymerase chain reaction (RT-PCR) was performed and demonstrated orosomucoid expression. After RT-PCR, the identities of the PCR products were confirmed by sequencing. RNase protection assay performed on total RNA from the HMVEC confirmed the results from the RT-PCR, i.e., orosomucoid mRNA is expressed by endothelial cells. Synthesis of orosomucoid in both liver and endothelial cells was demonstrated by immunoprecipitation. In conclusion, endothelial cells normally produce orosomucoid, which is essential for capillary charge selectivity. We suggest that orosomucoid exerts its effect by interacting with other components of the endothelial glycocalyx.
J Sörensson; G L Matejka; M Ohlson; B Haraldsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  276     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-30     Completed Date:  1999-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H530-4     Citation Subset:  IM    
Department of Physiology, Göteborg University, SE-405 30 Göteborg, Sweden.
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MeSH Terms
Capillary Permeability / physiology*
Cells, Cultured
Endothelium, Vascular / cytology,  metabolism*
Liver / metabolism
Orosomucoid / biosynthesis*,  genetics,  metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Orosomucoid; 0/RNA, Messenger

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