Document Detail


Human endothelial cells in culture produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) when stimulated with thrombin.
MedLine Citation:
PMID:  6587368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultured human endothelial cells produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF) when stimulated with human thrombin. The response to thrombin is dose dependent, with a half-maximal effect at 0.17 unit/ml. The product is identified as PAF by the incorporation of radiolabeled precursors, its behavior in chromatographic systems, the recovery of biological activity, and the effect of treatment with phospholipase A2. Incorporation of [3H]acetate into PAF is maximal by 5 min and decreases thereafter. Endothelial cells produce 0.10-0.17 nmol of PAF per 10(6) cells in a 5-min exposure to thrombin, as judged by the amount of neutrophil-aggregating activity. The production of this potent agonist for platelet activation and neutrophil chemotaxis by endothelial cells suggests that it may play a role in the maintenance of vascular integrity and perhaps in pathological events such as thrombosis and atherogenesis.
Authors:
S M Prescott; G A Zimmerman; T M McIntyre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  81     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-07-24     Completed Date:  1984-07-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3534-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid
Acetic Acids / metabolism
Cell Adhesion
Cells, Cultured
Endothelium / metabolism*
Granulocytes / physiology
Humans
Platelet Activating Factor / biosynthesis*
Platelet Adhesiveness
Thrombin / pharmacology*
Grant Support
ID/Acronym/Agency:
HL 00696/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Acetic Acids; 0/Platelet Activating Factor; 64-19-7/Acetic Acid; EC 3.4.21.5/Thrombin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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