Document Detail


Human embryo associated immunosuppressor factor(s) from pre- and post-implantation stages share some similarities.
MedLine Citation:
PMID:  2714849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study demonstrates that embryo associated immunosuppressive factor(s) (EASF) secreted by the human embryo at pre- and post-implantation stages share some similarities. Human EASF was partially purified from embryo growth media of in vitro fertilized ova and from first trimester pregnancy sera. Non-pregnancy sera were fractionated in parallel. During each step of purification the fractions were tested for immunosuppressive properties using concanavalin A-stimulated lymphocyte proliferation assay. Analysis of EASF positive fractions on SDS-PAGE identified 14 kDa and 24 kDa molecules in embryo growth media and pregnancy sera. No such molecules were found in control sera, suggesting that these factors are embryo associated. The relationship of pre- and post-implantation EASF was also analyzed by EASF binding assay using murine anti-EASF antibody, which was raised against EASF isolated from embryo growth media. Results show that murine antibody bound to EASF purified from pregnancy sera, but not to identical fractions from control sera, indicating that these post-implantation EASF possess some similarity with pre-implantation EASF. Results also indicate that a species of suppressor factors present in embryo growth media and pregnancy sera were unique for their origin. Presence of these three EASFs at various stages of gestation may play a role in suppressing maternal cellular immune responses thereby preventing maternal rejection of the embryo.
Authors:
R Bose
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology letters     Volume:  20     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-06-12     Completed Date:  1989-06-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  261-7     Citation Subset:  IM    
Affiliation:
Department of Obstetrics, Gynecology and Reproductive Sciences, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Descriptor/Qualifier:
Blastocyst / immunology*
Chromatography, Gel
Culture Media
Electrophoresis, Polyacrylamide Gel
Embryo, Mammalian / immunology*
Female
Humans
Lymphocyte Activation
Pregnancy
Radioimmunoassay
Suppressor Factors, Immunologic / blood,  isolation & purification*,  metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Suppressor Factors, Immunologic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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