| Human diploid fibroblast cells in senescence; cycling through polyploidy to mitotic cells. | |
| | |
MedLine Citation:
|
PMID: 16948503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Previously, it was found that senescent cells can undergo a modified cell cycle with mitotic cells as the end results. The major cycling events started with polyploidization, followed by depolyploidization to multinucleated cells (MNCs). These latter cells produced mononuclear offspring cells that could express mitotic cell divisions. In this report the emphasis is on late senescent fibroblasts that exhibited the senescence-associated change in cell morphology to large flat cells. Prior to live cell photography, flat cell cultures were maintained for months in the same culture flasks and therefore judged to be in a late senescent phase. All of the cellular events outlined above were present in these old cell cultures. Time lapse pictures showed movements of mitotic daughter cells away from each other and alignment of the chromosomes on the metaphase plate was visible in other mitotic cells. These data challenge the common view that cell senescence is irreversible and, therefore, an antitumor mechanism. A new finding was that the spike in polyploid cells in the near senescent phase consisted of cells with pairs of sister chromosomes from endoreduplication of DNA (two rounds of DNA synthesis and no mitosis). The lack of cells with 92 single chromosomes (e.g., G2 tetraploid cells) suggested that these polyploid cells also went through a changed cell cycle. The question now is whether these atypical polyploid cells are a subpopulation in senescence that can undergo the cycling from polyploidy to genome-reduced mitotic cells. |
| | |
Authors:
|
Kirsten H Walen |
Related Documents
:
|
1228333 - Nih gerontology research support: policy evaluation of in vitro cell lineage studies. 14506173 - Antisense-mediated htert inhibition specifically reduces the growth of human bladder ca... 2830153 - Nerve growth factor (ngf) induced differentiation of human neuroblastoma cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: In vitro cellular & developmental biology. Animal Volume: 42 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2006 Jul-Aug |
Date Detail:
|
Created Date: 2006-09-04 Completed Date: 2006-10-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: United States |
Other Details:
|
Languages: eng Pagination: 216-24 Citation Subset: IM |
Affiliation:
|
Viral and Rickettsial Disease Laboratory, California Department of Health Services, Richmond, CA 94801, USA. kwalen@dhs.ca.gov |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Aging
/
physiology* Cell Culture Techniques Cell Division / physiology Cells, Cultured Diploidy* Female Fibroblasts / cytology, physiology* Humans Microscopy, Phase-Contrast Mitosis / physiology* Polyploidy* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Heterogeneity amongst splenic stromal cell lines which support dendritic cell hematopoiesis.
Next Document: Effects of Ulmus davidiana planch on mineralization, bone morphogenetic protein-2, alkaline phosphat...