| Human dendritic cells expressing hepatitis C virus core protein display transcriptional and functional changes consistent with maturation. | |
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MedLine Citation:
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PMID: 20701679 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Summary. Hepatitis C virus (HCV) causes a chronic liver infection, which may result in cirrhosis and hepatocellular carcinoma. Impairment of the maturation process in dendritic cells (DCs) may be one of the mechanisms responsible for immune evasion of HCV. The core and NS3 proteins are among the most conserved HCV proteins and play a key role in viral clearance. To evaluate the effects of these proteins on DCs, monocyte-derived immature DCs (iDCs) were transfected with in vitro transcribed (IVT) HCV core or NS3 RNA and treated with maturation factors. Neither core nor NS3 had an inhibitory effect on DC maturation; however, transfection of iDCs with IVT core RNA appeared to result in changes compatible with maturation. To investigate this in more detail, the transcriptional profiles of iDCs transfected with IVT core, NS3 or green fluorescent protein (GFP) RNA were examined using a DC-specific membrane array. Of the 288 genes on the array, 46 genes were distinctively up- or down-regulated by transfection with IVT core RNA in comparison with NS3 or GFP RNA treatments. Forty-two of these genes are involved in DC maturation. The effects of core on maturation of iDCs were confirmed with a significant increase in surface expression of CD83 and HLA-DR, a reduction of phagocytosis, as well as an increase in proliferation and IFN-γ secretion by T cells in a mixed lymphocyte reaction assay. These results show that HCV core does not have an inhibitory effect on human DC maturation, but could be a target for the immune system. |
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Authors:
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A Landi; H Yu; L A Babiuk; S van Drunen Littel-van den Hurk |
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Publication Detail:
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Type: Journal Article Date: 2010-08-04 |
Journal Detail:
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Title: Journal of viral hepatitis Volume: 18 ISSN: 1365-2893 ISO Abbreviation: J. Viral Hepat. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9435672 Medline TA: J Viral Hepat Country: England |
Other Details:
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Languages: eng Pagination: 700-13 Citation Subset: IM |
Copyright Information:
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© 2010 Blackwell Publishing Ltd. |
Affiliation:
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Vaccine and Infectious Disease Organization, Saskatoon, SK, Canada Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Center for Disease Control, University of British Columbia, Vancouver, BC, Canada University of Alberta, 3-7 University Hall, Edmonton, AB, Canada Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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