Document Detail


Human dendritic cell differentiation pathway from CD34+ hematopoietic precursor cells.
MedLine Citation:
PMID:  8555475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The most effective antigen-presenting cells for T lymphocytes are dendritic cells (DCs), the differentiation pathway of which, however, is incompletely characterized. We examined here how DCs differentiated from human cord blood CD34+ progenitor cells cultured with granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and stem cell factor. After 5 days, 2 of 3 nonadherent cells were CD13hiHLA-DRhiCD4+, half of them were also CD14+, and < or = 10% were CD1a+. When day-5 sorted CD13hiCD1a- and CD13lo cells were further cultured, CD1a+ cells appeared in the already CD13hi population, whereas CD13hi cells, a minority of which rapidly became CD1a+, emerged from the CD13lo population. By day 12, still 66% of bulk cells in suspension were CD13hi, most of which displayed high forward and side scatters of large granular cells. Half of CD13hi cells were CD1a+. All CD13hi cells expressed to the same extent DR, CD4, costimulatory and adhesion molecules, and various amounts of CD14. CD1a+ cells stimulated allogeneic lymphocytes more than CD13hiCD1a- cells and, although they were CD14+, both cell types were nonspecific esterase-negative nonphagocytic cells and were stronger mixed leukocyte reaction stimulators than were their macrophage counterparts. Eventually, the percentage of CD1a+ cells decreased. However, typical CD1a+ DCs still emerged in culture of sorted day-12 CD13hiCD1a- cells, and adding interleukin-4 to bulk cultures at that time led to the persistence of the CD1a+ population while diminishing CD14 expression. Thus, this system results first in the differentiation of CD13hi precursors that strongly express DR and CD4, from which more mature CD1a+ DCs continuously differentiate all along the culture period.
Authors:
M Rosenzwajg; B Canque; J C Gluckman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Blood     Volume:  87     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-02-28     Completed Date:  1996-02-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  535-44     Citation Subset:  AIM; IM    
Affiliation:
Laboratoire de Biologie et Génétiques des Déficits Immunitaires, faculté de Médecine, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis
Antigens, CD34 / analysis
Biological Markers
Cell Differentiation / drug effects
Cells, Cultured
Dendritic Cells / cytology*
Fetal Blood / cytology
Hematopoietic Stem Cells / cytology*,  drug effects
Humans
Interleukin-4 / pharmacology
Lymphocyte Culture Test, Mixed
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD34; 0/Biological Markers; 207137-56-2/Interleukin-4

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