| Human dendritic cell differentiation pathway from CD34+ hematopoietic precursor cells. | |
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MedLine Citation:
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PMID: 8555475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The most effective antigen-presenting cells for T lymphocytes are dendritic cells (DCs), the differentiation pathway of which, however, is incompletely characterized. We examined here how DCs differentiated from human cord blood CD34+ progenitor cells cultured with granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and stem cell factor. After 5 days, 2 of 3 nonadherent cells were CD13hiHLA-DRhiCD4+, half of them were also CD14+, and < or = 10% were CD1a+. When day-5 sorted CD13hiCD1a- and CD13lo cells were further cultured, CD1a+ cells appeared in the already CD13hi population, whereas CD13hi cells, a minority of which rapidly became CD1a+, emerged from the CD13lo population. By day 12, still 66% of bulk cells in suspension were CD13hi, most of which displayed high forward and side scatters of large granular cells. Half of CD13hi cells were CD1a+. All CD13hi cells expressed to the same extent DR, CD4, costimulatory and adhesion molecules, and various amounts of CD14. CD1a+ cells stimulated allogeneic lymphocytes more than CD13hiCD1a- cells and, although they were CD14+, both cell types were nonspecific esterase-negative nonphagocytic cells and were stronger mixed leukocyte reaction stimulators than were their macrophage counterparts. Eventually, the percentage of CD1a+ cells decreased. However, typical CD1a+ DCs still emerged in culture of sorted day-12 CD13hiCD1a- cells, and adding interleukin-4 to bulk cultures at that time led to the persistence of the CD1a+ population while diminishing CD14 expression. Thus, this system results first in the differentiation of CD13hi precursors that strongly express DR and CD4, from which more mature CD1a+ DCs continuously differentiate all along the culture period. |
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Authors:
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M Rosenzwajg; B Canque; J C Gluckman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Blood Volume: 87 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1996 Jan |
Date Detail:
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Created Date: 1996-02-28 Completed Date: 1996-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 535-44 Citation Subset: AIM; IM |
Affiliation:
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Laboratoire de Biologie et Génétiques des Déficits Immunitaires, faculté de Médecine, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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analysis Antigens, CD34 / analysis Biological Markers Cell Differentiation / drug effects Cells, Cultured Dendritic Cells / cytology* Fetal Blood / cytology Hematopoietic Stem Cells / cytology*, drug effects Humans Interleukin-4 / pharmacology Lymphocyte Culture Test, Mixed |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD34; 0/Biological Markers; 207137-56-2/Interleukin-4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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