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Human β-defensin 2 expression in ELBW infants with severe necrotizing enterocolitis.
MedLine Citation:
PMID:  22902431     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background:The aim of this study was to analyse whether the mucosal innate immune response of extremely low birth weight (ELBW) infants might play a role in the development of necrotizing enterocolitis (NEC).Methods:Between 04/2008 and 12/2009 antimicrobial peptides were prospectively measured in fecal samples of ELBW infants. In cases requiring abdominal surgery, full thickness gut biopsies were analyzed for expression of human β-defensin 2 (hBD2), IL8, Villin, MD2, and Toll-like receptor 4 (TLR4).Results:Fecal hBD1 concentrations were consistently low in all patients whereas hBD2 concentrations were high in meconium particularly in clinical chorioamnionitis and then dropped, followed by a steady increase after day 14. Infants with moderate NEC showed significantly increased fecal hBD2 concentrations prior to clinical symptoms in contrast to infants developing severe NEC. Analysis of intestinal resection material obtained from patients with severe NEC revealed low hBD2 mRNA and protein levels with increased expression of the inflammatory cytokine IL-8.Conclusions:High hBD2 concentrations reflecting strong intestinal immune responses were associated with moderate courses of the disease. In severe NEC low hBD2 expression was accompanied by low TLR4/MD2 expression suggesting an inadequate response to luminal bacteria possibly predisposing those infants for the development of NEC.
Authors:
Andreas Jenke; Matthias Zilbauer; Jan Postberg; Stefan Wirth
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-17
Journal Detail:
Title:  Pediatric research     Volume:  -     ISSN:  1530-0447     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Neonatology, HELIOS Children´s Hospital, Witten/Herdecke University, 42283 Germany.
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