Document Detail

Human cytomegalovirus glycoprotein genotypes in the genital tract tissue of tubal pregnancy patients.
MedLine Citation:
PMID:  19383232     Owner:  NLM     Status:  MEDLINE    
This study investigated human cytomegalovirus (HCMV) glycoprotein genotypes in the genital tract tissue of 125 tubal pregnancy patients. The HCMV glycoprotein-B N-terminus (gBn, 54 - 485 NT), gB endoprotease cleavage site (gBclv, 1284 - 1600 NT) and glycoproteinH (gH, -58 - 213 NT) gene fragments were amplified by nested polymerase chain reaction and sequenced to identify gB and gH genotypes. Of 16 gBn-positive samples, four were gBn1, one was gBn2 and 11 were the gBn3 genotype. Of 13 positive gBclv samples, seven were gBclv1, two were gBclv2 and four were the gBclv3 genotype. Of 20 positive gH samples, 10 were gH1, six were gH2 and four were a combined gH1/gH2 genotype. In 10 of the samples that were positive for the gBn and gBclv genotypes, the gBn and gBclv genotypes were not consistent (four were gBclv1-gBn3). This study showed that: (i) HCMV infection with the gB1 - gB3 glycoprotein genotypes is present in tubal pregnancy; (ii) the gBclv and gBn genotypes are not strictly consistent; and (iii) intragenetic variability within the gB gene due to homologous recombination occurs frequently.
H-L Qian; T Cai; H-M Jin
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of international medical research     Volume:  37     ISSN:  0300-0605     ISO Abbreviation:  J. Int. Med. Res.     Publication Date:    2009 Mar-Apr
Date Detail:
Created Date:  2009-04-22     Completed Date:  2009-06-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0346411     Medline TA:  J Int Med Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  385-91     Citation Subset:  IM    
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
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MeSH Terms
Genitalia, Female / virology*
Polymerase Chain Reaction
Pregnancy, Tubal / virology*
Viral Envelope Proteins / genetics*
Reg. No./Substance:
0/Viral Envelope Proteins; 0/glycoprotein B, Simplexvirus; 0/glycoprotein N, Human cytomegalovirus

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