| Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 1717476 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have used immunofluorescence staining to study the subcellular distribution of cyclin A and B1 during the somatic cell cycle. In both primary human fibroblasts and in epithelial tumor cells, we find that cyclin A is predominantly nuclear from S phase onwards. Cyclin A may associated with condensing chromosomes in prophase, but is not associated with condensed chromosomes in metaphase. By contrast, cyclin B1 accumulates in the cytoplasm of interphase cells and only enters the nucleus at the beginning of mitosis, before nuclear lamina breakdown. In mitotic cells, cyclin B1 associates with condensed chromosomes in prophase and metaphase, and with the mitotic apparatus. Cyclin A is degraded during metaphase and cyclin B1 is precipitously destroyed at the metaphase----anaphase transition. Cell fractionation and immunoprecipitation studies showed that both cyclin A and cyclin B1 are associated with PSTAIRE-containing proteins. The nuclear, but not the cytoplasmic form, of cyclin A is associated with a 33-kD PSTAIRE-containing protein. Cyclin B1 is associated with p34cdc2 in the cytoplasm. Thus we propose that the different localization of cyclin A and cyclin B1 in the cell cycle could be the means by which the two types of mitotic cyclin confer substrate specificity upon their associated PSTAIRE-containing protein kinase subunit. |
| | |
Authors:
|
J Pines; T Hunter |
Related Documents
:
|
9737986 - Conserved cysteines in the type 1 deiodinase selenoprotein are not essential for cataly... 11545166 - Regulation of early embryo development: functional redundancy between cyclin subtypes. 10331086 - Cyclins and cell cycle checkpoints. 19686286 - Adenovirus vector-mediated upregulation of spermidine /spermine n1-acetyltransferase im... 21782986 - Saccharomyces cerevisiae in the stationary phase as a model organism - characterization... 7765096 - A novel ultrasonic resonance field device for the retention of animal cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of cell biology Volume: 115 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1991 Oct |
Date Detail:
|
Created Date: 1991-10-31 Completed Date: 1991-10-31 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1-17 Citation Subset: IM |
Affiliation:
|
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186-5800. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Transport CDC2 Protein Kinase / metabolism Cell Compartmentation Cell Cycle* Cell Line Cell Nucleus / metabolism Chromosomes / metabolism Cyclins / metabolism* Cytoplasm / metabolism Epitopes Fluorescent Antibody Technique Humans Metaphase Mitosis Mitotic Spindle Apparatus / metabolism Nuclear Proteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
CA-14195/CA/NCI NIH HHS; CA-39785/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cyclins; 0/Epitopes; 0/Nuclear Proteins; EC 2.7.11.22/CDC2 Protein Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Cell Biol Journal ID (publisher-id): J. Cell Biol. ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 10 Year: 1991 Volume: 115 Issue: 1 First Page: 1 Last Page: 17 ID: 2289910 Publisher Id: 92011851 PubMed Id: 1717476 |
| Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport | |
Article Categories:
|
|
Previous Document: The bonding behavior of calcite to bone.
Next Document: Paxillin is a major phosphotyrosine-containing protein during embryonic development.