Document Detail

Human collagen isolated from adipose tissue.
MedLine Citation:
PMID:  22549937     Owner:  NLM     Status:  Publisher    
Collagen, the most abundant protein in vertebrates, is a useful biomaterial in pharmaceutical and medical industries. So far, most collagen has been extracted from animals and cadavers. Herein, we suggest human adipose tissue, which is routinely abandoned after liposuction, as a plentiful source of human collagen. In this study, human collagen was obtained from adipose tissue through two successive major steps: i) extraction of the extracellular matrix (ECM) by pulverization, centrifugation, alkaline and alcohol treatment; ii) isolation of collagen from ECM by pepsin treatment in dilute acetic acid. The purified human adipose-derived collagen was characterized by FTIR spectroscopy, polyacrylamide gel electrophoresis, amino acid analysis, and circular dichroism spectroscopy. The extracted collagen showed a typical triple helix structure, good thermal stability due to abundant imino acids, and high solubility at acidic pH. The collagen greatly facilitated the adhesion and proliferation of human adipose-derived stem cells (HASC) and normal human dermal fibroblasts (NHDF) on polystyrene plates. These results suggest that human adipose tissue obtained by liposuction can provide human collagen for use in cosmetics, pharmaceutics, and medicine. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012.
Beob Soo Kim; Ji Suk Choi; Jae Dong Kim; Hwa In Yoon; Young Chan Choi; Yong Woo Cho
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-2
Journal Detail:
Title:  Biotechnology progress     Volume:  -     ISSN:  1520-6033     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 American Institute of Chemical Engineers (AIChE).
Department of Chemical Engineering and Department of Bionanotechnology, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
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