| Human chromosome 15 confers partial complementation of phenotypes to xeroderma pigmentosum group F cells. | |
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MedLine Citation:
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PMID: 2929593 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microcell-mediated transfer of a single human chromosome from repair-proficient human cells to genetic complementation group F cells from the hereditary disease xeroderma pigmentosum (XP) results in partial complementation of repair-defective phenotypes. The complementing chromosome was identified by cytogenetic and molecular analysis as human chromosome 15. Transfer of this chromosome to XP-F cells restores approximately 20% of the resistance of wild-type cells to killing by UV radiation or by the UV-mimetic chemical 4-nitroquinoline-1-oxide (4NQO), as well as partial repair synthesis of DNA measured as unscheduled DNA synthesis. Additionally, complemented XP-F cells have an enhanced capacity for reactivation of the plasmid-borne E. coli cat gene following its inactivation by UV radiation. Phenotypic complementation of XP cells by chromosome 15 is specific to genetic complementation group F; no effect on the UV sensitivity of XP-A, XP-C, or XP-D cells was detected. The observation that phenotypic complementation is partial is open to several interpretations and does not allow the definitive conclusion that the XP-F locus is carried on chromosome 15. |
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Authors:
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P J Saxon; R A Schultz; E J Stanbridge; E C Friedberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of human genetics Volume: 44 ISSN: 0002-9297 ISO Abbreviation: Am. J. Hum. Genet. Publication Date: 1989 Apr |
Date Detail:
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Created Date: 1989-05-03 Completed Date: 1989-05-03 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370475 Medline TA: Am J Hum Genet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 474-85 Citation Subset: IM |
Affiliation:
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Department of Pathology, Stanford University School of Medicine, CA 94305. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 15* DNA Damage DNA Repair Genetic Complementation Test Humans Phenotype Xeroderma Pigmentosum / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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CA-09151/CA/NCI NIH HHS; CA-19401/CA/NCI NIH HHS; CA-44247/CA/NCI NIH HHS |
| Comments/Corrections | |
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