Document Detail


Human chromosome 15 confers partial complementation of phenotypes to xeroderma pigmentosum group F cells.
MedLine Citation:
PMID:  2929593     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microcell-mediated transfer of a single human chromosome from repair-proficient human cells to genetic complementation group F cells from the hereditary disease xeroderma pigmentosum (XP) results in partial complementation of repair-defective phenotypes. The complementing chromosome was identified by cytogenetic and molecular analysis as human chromosome 15. Transfer of this chromosome to XP-F cells restores approximately 20% of the resistance of wild-type cells to killing by UV radiation or by the UV-mimetic chemical 4-nitroquinoline-1-oxide (4NQO), as well as partial repair synthesis of DNA measured as unscheduled DNA synthesis. Additionally, complemented XP-F cells have an enhanced capacity for reactivation of the plasmid-borne E. coli cat gene following its inactivation by UV radiation. Phenotypic complementation of XP cells by chromosome 15 is specific to genetic complementation group F; no effect on the UV sensitivity of XP-A, XP-C, or XP-D cells was detected. The observation that phenotypic complementation is partial is open to several interpretations and does not allow the definitive conclusion that the XP-F locus is carried on chromosome 15.
Authors:
P J Saxon; R A Schultz; E J Stanbridge; E C Friedberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  44     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-03     Completed Date:  1989-05-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  474-85     Citation Subset:  IM    
Affiliation:
Department of Pathology, Stanford University School of Medicine, CA 94305.
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Chromosome Banding
Chromosome Mapping
Chromosomes, Human, Pair 15*
DNA Damage
DNA Repair
Genetic Complementation Test
Humans
Phenotype
Xeroderma Pigmentosum / genetics*
Grant Support
ID/Acronym/Agency:
CA-09151/CA/NCI NIH HHS; CA-19401/CA/NCI NIH HHS; CA-44247/CA/NCI NIH HHS
Comments/Corrections

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