Document Detail

Human choriogonadotropin entrapped into liposomes: characterization, biologic effects and interaction with purified mouse Leydig cells in vitro.
MedLine Citation:
PMID:  2917687     Owner:  NLM     Status:  MEDLINE    
Liposomes containing human choriogonadotropin (hCG) were prepared from phosphatidylserine by the ether injection method. hCG adsorbed to the outer surface of the liposomes (77% of total liposome-associated hCG) was removed by proteolytic digestion with subtilisin. hCG-containing liposomes digested and not digested with subtilisin stimulated testosterone biosynthesis by Leydig cells in a dose-dependent way; both preparations had identical biologic activities (32% of the activity of free, not liposome-associated hCG) when equal doses of liposome-associated hCG were applied. The onset of stimulation was delayed when compared to the action of free hCG. Liposomes without hCG did not stimulate testosterone biosynthesis. Association of liposomes with Leydig cells was determined by measurement of transfer of radioactive label from liposomes to Leydig cells. The association was not mediated by the hormone receptor. hCG entrapped in liposomes was incorporated by Leydig cells and translated to the cellular surface. This process was impaired by colchicine (10(-5) M). hCG translocated to the external surface of the cell membrane contained a modified alpha-subunit (Mr 16,200 instead of 20,600) which was not detected in unentrapped hCG bound to Leydig cells. We suggest that liposomally entrapped hCG is taken up by Leydig cells and re-exported to the cell membrane by a mechanism resembling retroendocytosis.
A Lorenzen; W E Merz
Related Documents :
221237 - Cytotoxicity of ethyl methanesulfonate in mice spermatogonia.
7508977 - Effects of hormones, cyclic amp analogues and growth factors on steel factor (sf) produ...
2568857 - In vitro-perifused rat testes secrete beta-endorphin and dynorphin: their effect on tes...
7589787 - Possible involvement of microfilaments in the regulation of sertoli cell aromatase acti...
21455017 - Cell cycle suspension: a novel process lurking in g 2 arrest.
15506967 - Selective elimination of mitochondria from living cells induced by inhibitors of bioene...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  61     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-04-06     Completed Date:  1989-04-06     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  227-38     Citation Subset:  IM    
Department of Biochemistry II, University of Heidelberg, F.R.G.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Membrane / metabolism
Cells, Cultured
Chorionic Gonadotropin / metabolism*
Leydig Cells / metabolism*
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Liposomes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Peroxisomal oxidative capacity of brown adipose tissue depends on the thyroid status.
Next Document:  The N-terminal domain of the human androgen receptor is encoded by one, large exon.