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Human carcinoembryonic antigen, an intercellular adhesion molecule, blocks fusion and differentiation of rat myoblasts.
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MedLine Citation:
PMID:  8408226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human carcinoembryonic antigen (CEA), a widely used tumor marker, is a member of a family of cell surface glycoproteins that are overexpressed in many carcinomas. CEA has been shown to function in vitro as a homotypic intercellular adhesion molecule. This correlation of overproduction of an adhesion molecule with neoplastic transformation provoked a test of the effect of CEA on cell differentiation. Using stable CEA transfectants of the rat L6 myoblast cell line as a model system of differentiation, we show that fusion into myotubes and, in fact, the entire molecular program of differentiation, including creatine phosphokinase upregulation, myogenin upregulation, and beta-actin downregulation are completely abrogated by the ectopic expression of CEA. The blocking of the upregulation of myogenin, a transcriptional regulator responsible for the execution of the entire myogenic differentiation program, indicates that CEA expression intercepts the process at a very early stage. The adhesion function of CEA is essential for this effect since an adhesion-defective N domain deletion mutant of CEA was ineffective in blocking fusion and CEA transfectants treated with adhesion-blocking peptides fused normally. Furthermore, CEA transfectants maintain their high division potential, whereas control transfectants lose division potential with differentiation similarly to the parental cell line. Thus the expression of functional CEA on the surface of cells can block terminal differentiation and maintain proliferative potential.
Authors:
F J Eidelman; A Fuks; L DeMarte; M Taheri; C P Stanners
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  123     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-11-19     Completed Date:  1993-11-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  467-75     Citation Subset:  IM    
Affiliation:
McGill Cancer Centre, Biochemistry Department, McGill University, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Actins / analysis,  genetics,  physiology
Animals
Base Sequence
Blotting, Northern
Carcinoembryonic Antigen / analysis,  genetics,  physiology*
Cell Differentiation / physiology
Cell Division / physiology
Cell Fusion / physiology
Cell Line
Cell Transformation, Neoplastic / genetics,  metabolism
Creatine Kinase / analysis,  genetics,  physiology
DNA / genetics
Down-Regulation
Models, Biological
Molecular Sequence Data
Muscles / chemistry,  cytology*,  embryology*
Myogenin / analysis,  genetics,  physiology
Rats
Transfection
Up-Regulation
Chemical
Reg. No./Substance:
0/Actins; 0/Carcinoembryonic Antigen; 0/Myog protein, rat; 0/Myogenin; 9007-49-2/DNA; EC 2.7.3.2/Creatine Kinase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 2 Month: 10 Year: 1993
Volume: 123 Issue: 2
First Page: 467 Last Page: 475
ID: 2119830
Publisher Id: 94012990
PubMed Id: 8408226

Human carcinoembryonic antigen, an intercellular adhesion molecule, blocks fusion and differentiation of rat myoblasts


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