| Human carcinoembryonic antigen, an intercellular adhesion molecule, blocks fusion and differentiation of rat myoblasts. | |
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MedLine Citation:
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PMID: 8408226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human carcinoembryonic antigen (CEA), a widely used tumor marker, is a member of a family of cell surface glycoproteins that are overexpressed in many carcinomas. CEA has been shown to function in vitro as a homotypic intercellular adhesion molecule. This correlation of overproduction of an adhesion molecule with neoplastic transformation provoked a test of the effect of CEA on cell differentiation. Using stable CEA transfectants of the rat L6 myoblast cell line as a model system of differentiation, we show that fusion into myotubes and, in fact, the entire molecular program of differentiation, including creatine phosphokinase upregulation, myogenin upregulation, and beta-actin downregulation are completely abrogated by the ectopic expression of CEA. The blocking of the upregulation of myogenin, a transcriptional regulator responsible for the execution of the entire myogenic differentiation program, indicates that CEA expression intercepts the process at a very early stage. The adhesion function of CEA is essential for this effect since an adhesion-defective N domain deletion mutant of CEA was ineffective in blocking fusion and CEA transfectants treated with adhesion-blocking peptides fused normally. Furthermore, CEA transfectants maintain their high division potential, whereas control transfectants lose division potential with differentiation similarly to the parental cell line. Thus the expression of functional CEA on the surface of cells can block terminal differentiation and maintain proliferative potential. |
Authors:
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F J Eidelman; A Fuks; L DeMarte; M Taheri; C P Stanners |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of cell biology Volume: 123 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-11-19 Completed Date: 1993-11-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 467-75 Citation Subset: IM |
Affiliation:
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McGill Cancer Centre, Biochemistry Department, McGill University, Montreal, Quebec, Canada. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
/
analysis,
genetics,
physiology Animals Base Sequence Blotting, Northern Carcinoembryonic Antigen / analysis, genetics, physiology* Cell Differentiation / physiology Cell Division / physiology Cell Fusion / physiology Cell Line Cell Transformation, Neoplastic / genetics, metabolism Creatine Kinase / analysis, genetics, physiology DNA / genetics Down-Regulation Models, Biological Molecular Sequence Data Muscles / chemistry, cytology*, embryology* Myogenin / analysis, genetics, physiology Rats Transfection Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Carcinoembryonic Antigen; 0/Myog protein, rat; 0/Myogenin; 9007-49-2/DNA; EC 2.7.3.2/Creatine Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 2 Month: 10 Year: 1993 Volume: 123 Issue: 2 First Page: 467 Last Page: 475 ID: 2119830 Publisher Id: 94012990 PubMed Id: 8408226 |
| Human carcinoembryonic antigen, an intercellular adhesion molecule, blocks fusion and differentiation of rat myoblasts | |
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