Document Detail


Human brain aldolase C4 isoenzyme: purification, radioimmunoassay, and distribution in human tissues.
MedLine Citation:
PMID:  7406436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nervous system specific isoenzyme of frustose-1, 6-diphosphate aldolase (E.C.4.1.2.13), aldolase C4, has been purified from human brain, and a sensitive radioimmunoassay has been developed for its detection. This assay is also capable of detecting other hybrid isoenzymes containing the C subunit but not the A4 isoenzyme. A systematic survey of human organs has shown that immunoreactive aldolase C is present in all human organs but at levels less than 2% of those found in human brain; especially low levels occur in kidney, skeletal muscle, lung, and thyroid tissue. The presence of aldolase C in other organs apart from nervous tissue is unlikely to be explicable by innervation alone since significant quantities are found in erythrocytes. The high degrees of localisation of aldolase C4 in nervous tissue makes it a suitable marker for cell damage within the central nervous system.
Authors:
V J Willson; R J Thompson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of clinical biochemistry     Volume:  17     ISSN:  0004-5632     ISO Abbreviation:  Ann. Clin. Biochem.     Publication Date:  1980 May 
Date Detail:
Created Date:  1980-10-27     Completed Date:  1980-10-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0324055     Medline TA:  Ann Clin Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  114-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Brain / enzymology*
Erythrocytes / enzymology
Fructose-Bisphosphate Aldolase / analysis*,  blood,  isolation & purification
Humans
Isoenzymes / analysis*,  isolation & purification
Molecular Weight
Radioimmunoassay
Tissue Distribution
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 4.1.2.13/Fructose-Bisphosphate Aldolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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