| Human and bovine vascular endothelial cells. Comparative effect on cell growth and longevity of an eye-derived growth factor (EDGF) and of extracellular matrix. | |
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MedLine Citation:
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PMID: 6673799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human and bovine vascular endothelial cells from the umbilical vein and the aorta, respectively, were cultured in the presence of EDGF (a growth factor prepared from bovine retina) on plastic or on extracellular matrix (ECM). Both EDGF and ECM are required to allow the maximal proliferation of human cells and their organization in a typical monolayer. Conversely, bovine aortic endothelial cells grow perfectly in the absence of both factors in 6% fetal calf serum. However, a requirement for EDGF can also be demonstrated in low serum conditions, or in cells at high passage number. ECM had no growth promoting activity by itself. Thrombin acts similarly to EDGF on bovine serum-starved cells. EDGF prolongs the in vitro lifespan of both types of cells. Cells at all stages still synthesize factor VIII antigen as revealed by immunofluorescence. Thus EDGF, like other growth factors from brain, FGF or ECGF, may have an important role in angiogenesis, a critical problem in pathological retinas. |
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Authors:
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N Romquin; J Plouet; D Barritault; Y Courtois |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of the cell / under the auspices of the European Cell Biology Organization Volume: 49 ISSN: 0248-4900 ISO Abbreviation: Biol. Cell Publication Date: 1983 |
Date Detail:
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Created Date: 1984-06-06 Completed Date: 1984-06-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8108529 Medline TA: Biol Cell Country: FRANCE |
Other Details:
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Languages: eng Pagination: 109-18 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Vessels / cytology* Brain-Derived Neurotrophic Factor* Cattle Cell Differentiation / drug effects Cell Division / drug effects Cell Survival / drug effects Cells, Cultured Endothelium / cytology Extracellular Matrix Eye / metabolism Fibroblast Growth Factors Growth Substances / pharmacology* Humans |
| Chemical | |
Reg. No./Substance:
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0/Brain-Derived Neurotrophic Factor; 0/Growth Substances; 0/brain-derived growth factor; 0/eye-derived growth factor; 62031-54-3/Fibroblast Growth Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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