Document Detail


Human blood cells sialidases.
MedLine Citation:
PMID:  6477594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The conditions of a linear assay for 4-methylumbelliferyl-alpha-D-N-acetyl-neuraminic acid (4-MU-NeuAc) sialidase activity in human lymphocytes, granulocytes, platelets and red cells plasma membranes have been determined. Lymphocytes and granulocytes have the same pH curve with two maximums at pH 4.0 and 4.8; however lymphocytes show a higher specific activity and a higher Km value. Sialidase activity detected in red cells plasma membranes has again a double-peak pH curve with maximums at pH 4.2 and pH 4.6, and specific activity levels less than one/30 compared to the other blood cells preparations. Platelets have a sialidase activity of the same magnitude as granulocytes, with an optimum pH at 4.2.
Authors:
S Marchesini; B Cestaro; A Lombardo; G Sciorelli; A Preti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry international     Volume:  8     ISSN:  0158-5231     ISO Abbreviation:  Biochem. Int.     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-10-19     Completed Date:  1984-10-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8100311     Medline TA:  Biochem Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  151-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Platelets / enzymology*
Cell Membrane / enzymology
Erythrocyte Membrane / enzymology*
Granulocytes / enzymology*
Humans
Hydrogen-Ion Concentration
Hymecromone / analogs & derivatives,  metabolism*
Kinetics
Lymphocytes / enzymology*
Neuraminidase / blood*
Umbelliferones / metabolism*
Chemical
Reg. No./Substance:
0/Umbelliferones; 59322-44-0/2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid; 90-33-5/Hymecromone; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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